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pCSDest-HA-TMPRSS2

Plasmid

#154963

Purpose

TMPRSS2 with N-terminal HA

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

HA-TMPRSS2 (TMPRSS2 Human)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pExpreS2-1

Plasmid

#175360

Purpose

ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP

Plasmid

#62231

Purpose

Encodes hamster BiP/GRP78/HSPA5 fused to monomeric EGFP. Useful for studying BiP mobility and trafficking in live cells.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S

Plasmid

#108128

Purpose

Recruitable inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(C389S)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-Cyto-TurboID

Plasmid

#149414

Purpose

Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

Cyto-TurboID

Use

AAV

Tags

V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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TCARZ-CBh-GFP-SV40PA-GF3-R4A22

Plasmid

#203767

Purpose

For production of template RNA for use in PRINT, GFP under CBh promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Promoter

CBh

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMXspuro-GFP-NBR1

Plasmid

#74202

Purpose

Retroviral expression of GFP-NBR1

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

neighbor of BRCA1 gene 1 (NBR1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-Nck1

Plasmid

#45903

Depositor

Louise Larose

Article

Latreille et al J Biol Chem. 2006 Sep 8;281(36):26633-44

Insert

Nck-1 (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-HIF1 alpha K532R

Plasmid

#21102

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only
pLKO.1 puro GFP siRNA

Plasmid

#12273

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

GFP siRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Plasmid

#41863

Purpose

This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry2-ER

Plasmid

#164166

Purpose

Expresses mCherry2 fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2

Plasmid

#13755

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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PERK 1: PERK.WT.9E10.pCDNA

Plasmid

#21814

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pRSET FlipSuc-90u delta1

Plasmid

#14475

Depositor

Wolf Frommer

Article

Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

Insert

FlipThuE W283A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

N192D, W283A, 13 amino acids deleted from linkers

Promoter

T7

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV mouse Dscam

Plasmid

#18737

Depositor

Joshua Sanes

Article

Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

Insert

Dscam (Dscam Mouse)

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only
pBAF

Plasmid

#104152

Purpose

Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

Depositor

Robert Craigie

Article

Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

Insert

Barrier-to-autointegration factor (BAF) (BANF1 Human)

Tags

N-terminal His tag with thrombin cleavage site

Expression

Bacterial

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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ratGlp1-R

Plasmid

#14944

Depositor

Bernard Thorens

Article

Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

Insert

Glp1-R (Glp1r Rat)

Expression

Mammalian

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
Str-KDEL_flGalT-SBP-mCherry

Plasmid

#65273

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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