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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,310 results for: eng

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Showing: 4601 - 4620 of 14310 results

  11. pDIRECT_26G

    Plasmid
    #91149
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91149-plasmid-map-sequence-id-182283

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  12. pDIRECT_21B

    Plasmid
    #91129
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91129-plasmid-map-sequence-id-182252

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  13. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  20. pV1010

    Plasmid
    #111423
    Purpose
    gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)
    Depositor
    Gerald Fink
    Publication
    Vyas et al Sci Adv. 2015;1(3):e1500248.
    Insert
    sgADE-NT2
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111423-plasmid-map-sequence-id-212241

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Showing: 4601 - 4620 of 14310 results