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pFUW-Syb2-F77S

Plasmid

#248134

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with F77S mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-F77S (Vamp2 Rat)

Expression

Mammalian

Mutation

F77S

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-A67P

Plasmid

#248132

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with A67P mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-A67P (Vamp2 Rat)

Expression

Mammalian

Mutation

A67P

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-I45del

Plasmid

#248140

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with I45del deletion

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-I45del (Vamp2 Rat)

Expression

Mammalian

Mutation

I45del

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-Syb2-E78A

Plasmid

#248133

Purpose

Mammalian expression lentiviral vector encoding the wild-type Synaptobrevin2 (Syb2) with E78A mutation

Depositor

Ege Kavalali

Article

Guzikowski et al Proc Natl Acad Sci U S A. 2025 Nov 4;122

Insert

Syb2-E78A (Vamp2 Rat)

Expression

Mammalian

Mutation

E78A

Promoter

Ubiquitin

Available Since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Clover)-ScarlessDsRed

Plasmid

#244931

Purpose

Vector containing a Clover-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

Clover-tagged pYtag system (3xITAM P2A Clover-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-pYtag(Scarlet)-ScarlessDsRed

Plasmid

#244938

Purpose

Vector containing a Scarlet-based pYtag biosensor for CRISPR insertion into Drosophila RTKs, with a DsRed marker.

Depositor

Jared Toettcher

Article

Ho et al Cell Rep. 2025 Jul 22;44(7):115930. doi:

Insert

mScarlet-tagged pYtag system (3xITAM P2A mScarlet-ZtSH2)

Use

CRISPR

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTB105

Plasmid

#236778

Purpose

Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.

Depositor

Tom Beneke

Article

Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS1

Plasmid

#239460

Purpose

For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci. Adv., 11, eadw9153 (2025)

Type

Empty backbone

Tags

VENUS:3xHA:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMDS2

Plasmid

#239459

Purpose

Simultaneous monitoring of transcription and translation of genes in plant tissues: N- terminus fusion

Depositor

Miguel de Lucas

Article

Banda et al Sci. Adv., 11, eadw9153 (2025)

Type

Empty backbone

Tags

3xHA:VENUS:2A:mTurqN7

Expression

Bacterial and Plant

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DjCas13d-T2A-mCherry-pA

Plasmid

#192499

Purpose

To express DjCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-0.5Syn-CasRX-mCherry-pA

Plasmid

#192491

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.5Syn

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-0.5Syn-CasRx-pA

Plasmid

#192492

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.5Syn

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-T2A-mCherry-pA

Plasmid

#192494

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DjCas13d-pA

Plasmid

#192495

Purpose

To express DjCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-DIO-DjCas13d-pA

Plasmid

#192498

Purpose

To express DjCas13d in a Cre dependent manner

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

DjCas13d

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CasRx-pA

Plasmid

#192482

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-0.4CaMKII-CasRx-T2A-mCherry-pA

Plasmid

#192483

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

0.4CaMKIIa

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CasRx-T2A-mCherry-pA

Plasmid

#192484

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

Ef1a

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-intron-CasRx-pA

Plasmid

#192487

Purpose

To express CasRx

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV18

Plasmid

#237942

Purpose

Encodes TRBV18 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV18-TRAC (TRBV18 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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