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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 4621 - 4640 of 16276 results

  1. pACT1:Cas9-GFP, U6:sgUPRT

    Plasmid
    #122853
    Purpose
    Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene
    Depositor
    David Sibley
    Publication
    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9-GFP
    U6-sgUPRT
    Use
    CRISPR; Cas9-gfp plasmid for genome editing of cr…
    Tags
    eGFP
    Promoter
    Cryptosporidium parvum U6 and Cryptosporidium par…
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    122853-plasmid-map-sequence-id-238877

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  4. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  9. HOXD9 (human) pET

    Plasmid
    #8570
    Depositor
    Corey Largman
    Insert
    HOXD9 (HOXD9 Human)
    Tags
    T7
    Expression
    Bacterial
    Mutation
    PCR human cDNA clone; makes protein by in vitro T…
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only
    8570-plasmid-map-sequence-id-208949

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  10. eA3A-BE3 (pRZ206)

    Plasmid
    #131315
    Purpose
    CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    131315-plasmid-map-sequence-id-254673

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  14. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Available since
    Oct. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    68709-plasmid-map-sequence-id-125636

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  15. pIRES-mRFP-NLS-PCNA (2514)

    Plasmid
    #61850
    Purpose
    cell cycle marker
    Depositor
    Jonathon Pines
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA (PCNA Human)
    Tags
    NLS and mRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. EHMT1 (2IGQ)

    Plasmid
    #25504
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT1 (EHMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C-terminal domain, residues 951-1235
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article

  19. pOBCol3.6-GFPtpz

    Plasmid
    #110211
    Purpose
    Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.
    Depositor
    David Rowe
    Publication
    Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFPtopaz
    Tags
    None
    Expression
    Mammalian
    Mutation
    None
    Promoter
    3.6 kb rat type 1 collagen + first intron
    Available since
    June 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    110211-plasmid-map-sequence-id-211139

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Showing: 4621 - 4640 of 16276 results