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p2T-CMV-eA3AmaxNG-BlastR

Plasmid

#152999

Purpose

C-to-T base editor, NG PAM

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9 NG-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-26

Plasmid

#120005

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P06

Plasmid

#120017

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

sfGFP

Use

Pcr template

Tags

sfGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

Plasmid

#205261

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)

Plasmid

#179079

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMJA180

Plasmid

#128538

Purpose

Expresses E coli Bir A protein

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

E coli BirA

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Cx43-7

Plasmid

#58009

Purpose

Localization: Gap Junctions, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGLucIPZ

Plasmid

#228703

Purpose

Constitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interest

Depositor

Thorsten Stiewe

Article

Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1

Inserts

Gaussia luciferase
non-silencing shRNA

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 2, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 FLIPE-1m

Plasmid

#13544

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag FKHR H215R

Plasmid

#13509

Depositor

Kunliang Guan

Article

Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

Insert

FKHR H215R (FOXO1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H215R

Available Since

Dec. 11, 2006

Availability

Academic Institutions and Nonprofits only
pCAFNF-ECFP-Actb

Plasmid

#169787

Purpose

An actin FRET sensor. Must be expressed with Venus-actin for FRET.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Actb (Actb Mouse)

Tags

ECFP

Expression

Mammalian

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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LentiU6-hMYOD1 gRNA_1-MS2-Puro

Plasmid

#192673

Purpose

Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human MYOD1 activating gRNA #1 (MYOD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps54-V5-6His

Plasmid

#175098

Purpose

V5 tagged Vps54 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps54 (Vps54 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179064

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-VAM3

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE146

Plasmid

#118498

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

Plasmid

#25846

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA-W37I

Tags

Flag and His

Expression

Mammalian

Mutation

W37I

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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pEYFP_C1_FANCM

Plasmid

#221384

Purpose

Expresses FANCM in mammalian cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Fanconi anemia group M protein (FANCM Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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Twitch-1 pRSETB

Plasmid

#48207

Purpose

Fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Mues et al Nat Med. 2013 Jun;19(6):778-83. doi: 10.

Insert

Twitch-1

Tags

6xHIS, ECFP, and cpCit174

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only
pEE491

Plasmid

#149292

Purpose

T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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