We narrowed to 9,772 results for: Tec;

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pBabe-Myc-TRF1iDDR

Plasmid

#44576

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

chimeric TRF (TERF2, TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

TRF1 containing the iDDR domain of TRF2 (aa407-43…

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-PRKAA2ΔC

Plasmid

#30307

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α2 ΔC (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids Ser 527 to Arg 552

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
ATF4 12: 5’ATF4.uORF1AUA.GFP

Plasmid

#21859

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF1 was converted to AUA

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pU1utr-EGFP

Plasmid

#140268

Purpose

U1A-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

U1A binding site

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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plet7d_stbC-EGFP

Plasmid

#140266

Purpose

LIN28-dependent translational repression

Depositor

Hirohide Saito

Article

Kawasaki et al Nucleic Acids Res. 2017 May 19. doi: 10.

Insert

Stabilized hairpin of miR-let7d

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-Hs4E-BP3

Plasmid

#79439

Purpose

Expresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cells

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4E-BP3 (EIF4EBP3 Human)

Tags

lambdaN-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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LLP777_SnoopCatcher-p65HSF1-CO

Plasmid

#211770

Purpose

SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-p65HSF1

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-EGFP MASTL G44S (siRNA resistant)

Plasmid

#191012

Purpose

Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

Depositor

Johanna Ivaska

Article

Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Microtubule-associated serine/threonine kinase like (MASTL Human)

Tags

EGFP

Expression

Mammalian

Mutation

G44S

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mcherry-p62 delta PB1 silent

Plasmid

#187985

Purpose

human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Lacking PB1 domain (aa1-102) with a silent mutati…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-eDHFR(69K6)-MCS

Plasmid

#172100

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-20aa

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1beta

Plasmid

#181901

Purpose

Fluorescently tagged HP1beta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1beta (Cbx1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Tollip M240A,F241A

Plasmid

#178051

Purpose

GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Methionine 240 to Alanine and changed Phe…

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCIBN-TetR

Plasmid

#103813

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR (CIB1, tetR Mustard Weed, E. coli)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

Plasmid

#69842

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M-S285A-T289A

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL14-GFP

Plasmid

#67406

Purpose

Mammalian expression of hArl14 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Tags

GFP

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pT7-agrBD-IV

Plasmid

#53440

Purpose

Derivative of pT7-agrBD-I with a point mutation in the agrD gene to change AIP coding region from type-I to type-IV

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus (with D29Y mutation in agrD)

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

D to Y mutation of residue 29 of the agrD gene (c…

Promoter

T7-lac

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTRCHisB-TRF2∆M

Plasmid

#50489

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Delta Myb (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-437

Promoter

pTrc

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTRCHisB-TRF2_M

Plasmid

#50490

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Myb (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 438-500

Promoter

pTrc

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-Myc-TRFcH

Plasmid

#44574

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

chimeric TRF (TERF2, TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

TRF2 Hinge domain (aa245-443) replaced by that of…

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-CD86-mApple

Plasmid

#133860

Purpose

Mammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mApple

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

CD86 (CD86 Human)

Tags

EGFP and mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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