We narrowed to 5,870 results for: SPI;
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Plasmid#64336PurposeThis a retroviral expression plasmid expressing GFP along with puro resistance geneDepositorInsertGFP
UseRetroviralTagsGFPAvailable SinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc LbaCas12a
Plasmid#182123PurposepET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc LbaCas12a
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T305D/T306D)
Plasmid#127611PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
TagsmEGFPExpressionMammalianMutationchanged Threonine 305/306 to AlaninePromoterpCAGAvailable SinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1
Plasmid#45822DepositorInsertAS9(Mito MTS)-Delta N67-mMCL1
ExpressionMammalianMutationATP Synthase subunit 9 is used as a Mito Targetin…Available SinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
AfrLEA3m_mCh-2xFKBP (pBS1137)
Plasmid#185321PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertAfrLEA3m
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_APOE_HUMAN_E4_47-300 (pBS0847)
Plasmid#185279PurposeFor the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAPOE_HUMAN_E4_47-300
ExpressionMammalianAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-CheRiff-EGFP
Plasmid#114108Purposefusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1-6xHis-TEV-Nsp8
Plasmid#201022PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
Tags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHEN-CoV2-nanobody-Ty1
Plasmid#194588PurposeBacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertTy1 (S )
ExpressionBacterialAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAHS2parrc-mCh-2xFKBP (pBS1109)
Plasmid#185304PurposeFor the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS2parrc
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1(long)-His6
Plasmid#69110PurposeFor bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.DepositorAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Topo_SpCas9.tracr_LbCas12a.DR
Plasmid#155049PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
UseCRISPRAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRY-tTA (B702)
Plasmid#92032PurposeExpresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activatorDepositorInsertCRY2 (CRY2 Mustard Weed)
TagstTA2 (TetR fused to VP64)ExpressionMammalianMutationNLS on CRY2 is mutatedPromoterCMVAvailable SinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2-coHPDL WT 3xFlag-V5
Plasmid#174129PurposeExpresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cellsDepositorInsert4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
UseLentiviralTags3xFLAG-V5ExpressionMammalianMutationCodon optimizedAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA_81-236-FLAG (pBS0297)
Plasmid#185328PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA_81-236-FLAG
TagsFLAGExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PH-SWAP70(R223E, R224E)
Plasmid#84583PurposeExpresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide bindingDepositorInsertSWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
TagsEGFPExpressionMammalianMutationPleckstrin-homology domain, residues 209-306, mut…PromoterCMVAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGY466
Plasmid#166661PurposeFor light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeastDepositorInsertLiCre
UseCre/LoxExpressionYeastMutationCre: E340A, D341APromoterMET17Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-α-COPI-WD40
Plasmid#180758PurposeMammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)DepositorInsertα-COPI-WD40
TagsC-terminal strep tagExpressionMammalianMutationEncodes residues 1-327; mutations: Leu181Lys, Leu…PromoterCMVAvailable SinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1 S708/710A
Plasmid#70153Purposemammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutationsDepositorAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp4-a del.SHD
Plasmid#40067DepositorInserthSlp4-a V21A (SYTL4 Human)
TagsGSTExpressionBacterialMutationdeleted SHD domain (1-143)PromoterTacAvailable SinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only