We narrowed to 81,643 results for: TRI

1. pCHC005 (∆RE)

   Plasmid

   #226199

   Purpose

   Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for type I restriction-modification system restriction subunit H16_A0006
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. Twitch-4 pcDNA3

   Plasmid

   #49533

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-4
   

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGEX-6P-1-HsRCK_E

   Plasmid

   #146210

   Purpose

   Bacterial Expression of HsRck

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck (DDX6 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p5M-hPR-B

   Plasmid

   #89114

   Purpose

   mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

   Depositor

   Elizabeth Wilson

   Article

   Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

   Insert

   human progesterone receptor-B (PGR Human)
   

   Expression

   Mammalian

   Mutation

   5 restriction sites deleted in polylinker

   Promoter

   CMV

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CMV-H2B-3xFLAG-2A-G(N2c)

   Plasmid

   #194355

   Purpose

   AAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strain

   Depositor

   Marco Tripodi

   Article

   Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

   Inserts

   H2B-3xFLAG
   Rabies CVS-N2c glycoprotein (G)
   

   Use

   AAV

   Tags

   FLAG

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEYFP-N1-eKR2

   Plasmid

   #115337

   Purpose

   Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

   Depositor

   Peter Hegemann

   Article

   Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

   Insert

   eKR2
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCA24N-ligase-cTF

   Plasmid

   #87743

   Purpose

   IPTG-inducible expression of ligase-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Tags

   6xHis and cTF (chimeric transcription factor base…

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMuLE_ENTR_CBF-GLuc_L3-L2

   Plasmid

   #113868

   Purpose

   Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CBF (notch dependent promoter)
   GLuc
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. gRNA-leu-HYB

   Plasmid

   #64332

   Purpose

   leu2 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of leu2 deletion gRNA cassette
   

   Expression

   Yeast

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pMXS-IRES-Blast coUGP2

    Plasmid

    #211531

    Purpose

    Expresses codon optimized UGP2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UGP2 (UGP2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

    Plasmid

    #202644

    Purpose

    Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

    Depositor

    Balázs Enyedi

    Article

    Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

    Insert

    PM-mKate2-P2A-GEM-LTB4mut
    

    Tags

    PM-mKate2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTK47_mCherry-LGN-hardened

    Plasmid

    #46347

    Purpose

    rescues the phenotypes caused by LGN RNAi

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    LGN (GPSM2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

    Promoter

    CMV

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. L2_UBE2:mT-N7-CsA

    Plasmid

    #136133

    Purpose

    Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpUBE2:mTurq-N7
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGREAT2

    Plasmid

    #170890

    Purpose

    GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    p35SoTMV

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Twitch-1 pcDNA3

    Plasmid

    #49812

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-1
    

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

    Plasmid

    #113864

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    PTCH1 (hedgehog dependent promoter)
    mTurquoise2
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV4a-SIRT6-H133Y-FLAG

    Plasmid

    #102324

    Purpose

    Human SIRT6 H133Y with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG_EGFP-RandE3

    Plasmid

    #79870

    Purpose

    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Promoter

    Beta-actin

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Promoter

    EF1a

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. gRNA-his-HYB

    Plasmid

    #64333

    Purpose

    his3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of his3 deletion gRNA cassette
    

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only