We narrowed to 10,236 results for: ada

1. pAM4951 (pCV0063)

   Plasmid

   #120084

   Purpose

   Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

   Depositor

   Susan Golden

   Article

   Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

   Insert

   Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
   

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF6 VPS51-V5-6His

   Plasmid

   #175502

   Purpose

   V5 tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

   Insert

   VPS51 (VPS51 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   A661V- please see depositor comments

   Available Since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hPPP3R1

   Plasmid

   #179136

   Purpose

   Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HMGA2-FLAG

   Plasmid

   #25409

   Depositor

   Sean Morrison

   Article

   Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

   Insert

   high mobility group AT-Hook 2 (Hmga2 Mouse)
   

   Use

   Retroviral; Chip

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   None

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196079

   Purpose

   Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196085

   Purpose

   Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. px458_2A_GFP_sgRNA_G3BP2_G1

   Plasmid

   #127112

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA G3BP2 (G3BP2 Human)
   

   Use

   CRISPR

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE FLAG-IKK2-EE puro

   Plasmid

   #216790

   Purpose

   Mammalian expression of constitutively active human IKK-2

   Depositor

   Kevin Janes

   Article

   Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

   Insert

   IKK-2 (IKBKB Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S177E and S181E, constitutively active

   Promoter

   LTR

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1-IFT54

   Plasmid

   #218726

   Purpose

   Expresses N-terminally EGFP-tagged IFT54 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Hiyamizu et al J Cell Sci. 2023 Mar 1;136(5):jcs260462.

   Insert

   IFT54 (TRAF3IP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHeCAHS-mEGFP-NLS

    Plasmid

    #205014

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the chas gene from H. exemplaris
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1P shFXN_1

    Plasmid

    #102970

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_1 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T-1-β4 hinge-ear

    Plasmid

    #196692

    Purpose

    Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Human, Synthetic)
    

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_APOBEC1-UdgX-nCas9

    Plasmid

    #163531

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-DONSON

    Plasmid

    #208797

    Purpose

    Xenopus laevis His6-Myc-DONSON wild type

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Tags

    His and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159858

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-FLAG-RIPK3 K50A

    Plasmid

    #78817

    Purpose

    To overexpress RIPK3 K50A in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K50A

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. LifeAct-mAvicFP1

    Plasmid

    #129512

    Purpose

    Expresses LifeAct-mAvicFP1 in mammalian cells

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    LifeAct-mAvicFP1
    

    Expression

    Mammalian

    Mutation

    mAvicFP1 is AvicFP1 F64L + A206K

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1P shFXN_2

    Plasmid

    #102971

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_2 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. NO- DSD Sensor 3

    Plasmid

    #199787

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    NO- DSD Sensor
    

    Use

    Synthetic Biology

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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