We narrowed to 9,954 results for: ada

1. pMKO.1 puro-luc (shRNA)

   Plasmid

   #153962

   Purpose

   Expresses a shRNA against firefly luciferase

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   shRNA against firefly luciferase (19-bp-long target)
   

   Use

   Luciferase, RNAi, and Retroviral

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-N-mSOG-bFos-IRES-mCherry

   Plasmid

   #136599

   Purpose

   Encodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   N-mSOG-bFos
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IPU-FLAG-NEK9T210A

   Plasmid

   #168270

   Purpose

   Expresses kinase-dead NEK9 mutant in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed threonine 210 to alanine

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST_AfrLEA3m (pBS0911)

   Plasmid

   #185285

   Purpose

   For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   AfrLEA3m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST_PvLEA4_repeats_k3_26 (pBS0762)

   Plasmid

   #185244

   Purpose

   For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k3_26
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFJ2441 - [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159870

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-Dendra2(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-mEGFP-mTrim21ΔPRYSPRY

   Plasmid

   #105521

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR RPS27A K113R-3xFlag_IRES-GFP

   Plasmid

   #198391

   Purpose

   RPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   RPS27A (RPS27A Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   ubiquitination site mutant (K113R)

   Promoter

   EF1A

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRvCAHS1-mEGFP-NLS

   Plasmid

   #205013

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRvSAHS1-mCherry-NLS

    Plasmid

    #205009

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPR v2-sgAMPK alpha1 clone2

    Plasmid

    #162120

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p7612 MSCV-P C-FlagHA 16E7 E10K

    Plasmid

    #163303

    Purpose

    Expresses HPV16 E7 E10K

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 E10K
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    E10K

    Promoter

    MSCV LTR

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFN-Tel-TAL/pCMV-7.1

    Plasmid

    #63589

    Purpose

    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

    Insert

    3xFVN-Tel-TAL
    

    Use

    TALEN

    Tags

    3xFLAG-tag, V5-tag, and nuclear localization sign…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-TPI_PTC160-xrRNA-4H

    Plasmid

    #108369

    Purpose

    Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PTC160

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 7749 Bicistronic_ires_hygro

    Plasmid

    #64374

    Purpose

    This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEX-YFP-hLC3ΔG

    Plasmid

    #24990

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a mutant human MAP1-LC3B fused with EYFP (MAP1LC3B Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    deleted Gly at 121

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pST_SERF2_HUMAN (pBS0751)

    Plasmid

    #185239

    Purpose

    For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    SERF2_HUMAN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX6P1 UBXN11

    Plasmid

    #169017

    Purpose

    Expresses GST-UBXN11 (human) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    UBXN11 (UBXN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. -111 hTF m2

    Plasmid

    #15449

    Depositor

    Nigel Mackman

    Article

    Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    All 3 Sp1 sites mutated

    Promoter

    Available since

    Aug. 31, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid

    #159861

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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