We narrowed to 10,326 results for: ada

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pPB12A

Plasmid

#210035

Purpose

Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas12a
rtta-T2A-puro

Use

CRISPR

Tags

nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV10-Flag-Fbxl21

Plasmid

#48752

Purpose

Expression of FLAG-tagged Fbxl21

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Fbxl21 (Fbxl21 Mouse)

Tags

3XFLAG

Expression

Mammalian

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p37-Strep-HA

Plasmid

#113485

Purpose

Expression of human p37 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

p37 (UBXN2B Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-2

Plasmid

#72344

Purpose

Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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MAC_N_NFIB

Plasmid

#167783

Purpose

Expresses NFIB in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

NFIB (NFIB Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-MYO1C

Plasmid

#227305

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of MYO1C (MYO1C Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-nCas9

Plasmid

#163535

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OTmut

Plasmid

#185389

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-2XFLAG,2XHA-CyclinF-puro

Plasmid

#32976

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pL6-H2B-iRFP720-puro

Plasmid

#189992

Purpose

Lentivirial plasmid for nuclear iRFP-720 expression with puromycin resistance

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Human histone H2B

Use

Lentiviral

Tags

iRFP720

Expression

Mammalian

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

Plasmid

#136603

Purpose

Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

Depositor

John Ngo

Article

Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

Insert

FKBP-C-mSOG-J-alpha

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHR RPS27A-3xFlag_IRES-GFP

Plasmid

#198390

Purpose

RPS27A (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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p221z-erVenusYFP

Plasmid

#71265

Purpose

Entry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

VenusYFP

Use

Gateway

Tags

ER retention signal HDEL and signal peptide (ER)…

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-PXN

Plasmid

#227316

Purpose

Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

PXN Homology Arms flanking a mStayGold Tag (PXN Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-rnSOX9 PQA/S (286-507)

Plasmid

#62975

Purpose

Expresses HA-tagged rat SOX9 residues 286-507

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

SOX9 (Sox9 Rat)

Tags

HA (YPYDVPDY)

Expression

Mammalian

Mutation

codes for residues 286-507

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA SF-HERC2 F1

Plasmid

#55784

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pCDNAZeo(-)HSC73

Plasmid

#86030

Purpose

Expression of Heat shock chaperone 70 in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Heat-shock cognate protein 73 (HSPA8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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p221z-erTagRFP

Plasmid

#71266

Purpose

Entry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

TagRFP

Use

Gateway

Tags

ER retention signal HDEL and signal peptide (ER)…

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMDJ112 - pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159811

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 | eFlp recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mScarlet-H2BC11

Plasmid

#207756

Purpose

Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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