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Plasmid#115930PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/5 SGP NA (No Aptamer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW083
Plasmid#115931PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/14 SGP NA (No Aptamer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn2 (CG6812)
Plasmid#110643PurposeRetroviral expression of Flag-tagged Drosophila CG6812 in mammalian cellsDepositorInsertCG6812 (CG6812 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-PARK6e5-I368N
Plasmid#86154PurposeDonor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomatoDepositorInsertPINK1 exon 5 homology arms (PINK1 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hSesn2 R419A/K422A/K426A
Plasmid#111793PurposeMammalian expression or 3rd generation lentivirus generation of hSesn2DepositorInserthSesn2 (SESN2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR419A/K422A/K426APromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hSesn2 L373A/N376A/D385A/S387A
Plasmid#111789PurposeMammalian expression or 3rd generation lentivirus generation of hSesn2DepositorInserthSesn2 (SESN2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationL373A/N376A/D385A/S387APromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hSesn2 R404A/D406A/D407A
Plasmid#111790PurposeMammalian expression or 3rd generation lentivirus generation of hSesn2DepositorInserthSesn2 (SESN2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR404A/D406A/D407APromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-Flag-hSesn2 D409A/G411A/Q415A
Plasmid#111791PurposeMammalian expression or 3rd generation lentivirus generation of hSesn2DepositorInserthSesn2 (SESN2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationD409A/G411A/Q415APromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-dCEN-Incenp
Plasmid#108479Purposeexpression of vsv-dCEN-INCENPDepositorInsertdCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box removedPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104619PurposeExpresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4DepositorInsert3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PatoM-OG241
Plasmid#72842PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.DepositorInsertPatoM
UseTagsExpressionBacterialMutationPromoterPatoMAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment B
Plasmid#86659PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated by cleavage at the KpnI site from 880 (…PromoterCDK7Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4F07
Plasmid#71056PurposeGateway entry cloneDepositorInsertMi-2 (Mi-2 Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF1G05
Plasmid#70809PurposeGateway entry cloneDepositorInsertCkIIbeta (CkIIbeta Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF1B07
Plasmid#70819PurposeGateway entry cloneDepositorInsertCkIIalpha (CkIIalpha Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E,NoCys)
Plasmid#66875PurposeExpresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1 (S160E) with all Cys to Ser mutations
UseTagsHistagExpressionBacterialMutationS160E; changed 4 (all) cysteines to serinePromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-HKII-F
Plasmid#64518PurposeAS-30D hepatoma hexokinase II 0.075 kbp exon I-luciferase reporter vectorDepositorInsertType II hexokinase gene, partial cds and promoter region (Hk2 Rat)
UseLuciferaseTagsfirefly luciferaseExpressionMutationIsolated from AS-30D rat hepatomaPromoterHexokinase II promoter deletedAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFN-Tel-TAL/pMXs-neo
Plasmid#63591PurposeExpresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene.DepositorInsert3xFVN-Tel-TAL
UseRetroviral and TALENTags3xFLAG-tag, V5-tag, and nuclear localization sign…ExpressionMammalianMutationPromoterLTRAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycExpressionMutationlacks E3 domain on N-term (del aa1-274)PromoterAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-0X750-luxAB-DAS+4
Plasmid#49519PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomallyDepositorInsertP750-luxAB-DAS+4
UseTagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialMutationPromoterP750Available sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125L-siResist
Plasmid#49855PurposeEncodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100 and Methionine 125 mutated to Leuci…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100L-siResist
Plasmid#49854PurposeEncodes human connexin 43 with M100 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationMethionine100 mutated to Leucine. Contains severa…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant 4
Plasmid#45387DepositorInsert6 copies of hPAI-1 promoter -732/-721 four point mutation (SERPINE1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationmutated from agacaaggttgt to acactaggatgaPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
Comp AlphaPS3
Plasmid#14071DepositorInsertDrosphila Comp Alpha PS3 Integrin (scb Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL(aa23-340) D320N-His
Plasmid#111289Purposebacterial expressionDepositorInserthuman CLYBL ( aa23-340) D320N codon optimized for bacterial expression (CLYBL Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
MG1655 ΔendA ΔrecA (DE3)
Bacterial Strain#37854DepositorBacterial ResistanceNoneAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
Golden Gate TALEN and TAL effector kit 2.0
Plasmid Kit#1000000024PurposeUsed to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.DepositorAvailable sinceJan. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(F74G)-V5
Plasmid#158664PurposeTet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(F74G) AAVS1
UseCRISPRTagsExpressionMammalianMutationF74GPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSB-KAN
Plasmid#166700PurposeCombined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA Ser promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSB-HYG
Plasmid#166699PurposeCombined sgRNA/Cas9 pLSB vector with hphMX6 (hygromycin) marker. Golden Gate-ready for cloning custom sgRNA sequences.DepositorInsertstRNA promoter : sgRNA cassette
adh15 promoter : Cas9 codon-optimised for S. pombe
UseTagsExpressionYeastMutationPromoteradh15 promoter and tRNA promoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only