We narrowed to 152,820 results for: DES

1. pCDH_Str-KDEL_neomycin

   Plasmid

   #65307

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Use

   Lentiviral

   Tags

   KDEL motif and Signal Sequence

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBR_Cas9_sp1

   Plasmid

   #154851

   Purpose

   Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

   Depositor

   John van der Oost

   Article

   Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

   Inserts

   Spcas9 gene
   sgRNA scaffold
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Mutation

   Codon harmonized variant for expression in Rhodob…

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Grp1-PH pGEX2TK

   Plasmid

   #71379

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

   Insert

   Grp1-PH (Cyth3 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 264-391

   Promoter

   tac

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Str-KDEL_ManII-SBP-EGFP

   Plasmid

   #65252

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin-KDEL and truncated Mannosidase II
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ii-Str_TNF-SBP-EGFP

   Plasmid

   #65280

   Purpose

   synchronize trafficking of TNF from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-DeltaFosB

   Plasmid

   #68545

   Purpose

   AAV expression of DeltaFosB

   Depositor

   Eric Nestler

   Article

   Zachariou et al Nat Neurosci. 2006 Feb;9(2):205-11. Epub

   Insert

   DeltaFosB-IRES-GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   truncated splice variant of FosB

   Promoter

   CMV

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pMBEC8

   Plasmid

   #187599

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-opto-mRipK3

   Plasmid

   #208783

   Purpose

   Inducibly expresses mouse RipK3 fused to light activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   mCherry mRipK3 Cry2 (Ripk3 Mouse)
   

   Use

   Lentiviral

   Tags

   Cry2 and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-DeltaJunD

   Plasmid

   #68547

   Purpose

   AAV expression of DeltaJunD

   Depositor

   Eric Nestler

   Article

   Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

   Insert

   DeltaJunD-IRES-GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   truncated splice variant

   Promoter

   CMV

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

10. GRK2-K220R

    Plasmid

    #35403

    Depositor

    Robert Lefkowitz

    Article

    Freedman et al J Biol Chem. 1995 Jul 28;270(30):17953-6

    Insert

    GRK2 (GRK2 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K220R

    Promoter

    CMV

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. HUWE1-sgRNA-1

    Plasmid

    #86924

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA1 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

12. HUWE1-sgRNA-2

    Plasmid

    #86925

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA2 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

13. PT2/C-fluc

    Plasmid

    #20203

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    C-Fluc
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCit-APPAPAPPAP-gs

    Plasmid

    #228091

    Purpose

    Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells

    Depositor

    Roman Jerala

    Article

    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

    Insert

    mCit, APPAPAPPAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-h-mmPtch1-FL

    Plasmid

    #120889

    Purpose

    Expresses wildtype Ptch1 for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ii-Str_ManII-SBP-mCherry

    Plasmid

    #65256

    Purpose

    synchronize trafficking of ManII from the ER

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ii-Streptavidin and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-mNectarine

    Plasmid

    #21717

    Purpose

    Bacterial expression of pH-sensitive monomeric mNectarine red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Johnson et al J Biol Chem. 2009 Jul 31;284(31):20499-5

    Insert

    mNectarine fluorescent proteins
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    The fluorescent protein was inserted between XhoI…

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. SG3ΔENV RT D185A

    Plasmid

    #145782

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Reverse Transcriptase
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Reverse Transcriptase Aspartic Acid 185 t…

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT/CMV-SV40-LgT

    Plasmid

    #20204

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    SV40LgT
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge