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pmMaple2-CAM

Plasmid

#101149

Purpose

Fluorescent protein for superresolution imaging

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

E. coli codon optimized mMaple2

Expression

Bacterial

Promoter

pBAD

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-GFP1

Plasmid

#106422

Purpose

Y-RAN-GFP1 (RANbody GFP1::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP1

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD mPlum

Plasmid

#91773

Purpose

Bacterial expression of a fluorescent protein, monomeric Plum (mPlum)

Depositor

Erik Rodriguez, Roger Tsien

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Insert

monomeric Plum

Tags

no tag

Expression

Bacterial

Promoter

araBAD

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-GG

Plasmid

#182154

Purpose

Expression of artificial intron separated eGFP

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

GG

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2-eab2-AA

Plasmid

#182153

Purpose

Expression of artificial intron separated mApple

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

AA

Use

Zebrafish expression

Promoter

eab2

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-SUMO-KaiA

Plasmid

#68049

Purpose

Strain: AM5115, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiA

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-SUMO-KaiB

Plasmid

#68050

Purpose

Strain: AM5116, Used for protein purification

Depositor

Susan Golden

Article

Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

Insert

KaiB

Tags

6xHis and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAndy-NBioTAP

Plasmid

#53571

Purpose

Plasmid vector carrying N-Bio-1xproteinA tag

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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Venus Cam KII alpha mutant T286A

Plasmid

#29430

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Mutation

T286A mutation in CamKIIa sequence

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pBI121-Pro-Adh::TaPCS1:cmyc

Plasmid

#49765

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in plant expression vector

Depositor

Julian Schroeder

Article

Gong et al Proc Natl Acad Sci U S A. 2003 Aug 19;10

Insert

TaPCS1 (PCS1 Triticum aestivum)

Use

Plant expression

Tags

c-myc

Promoter

Adh1

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
library backbone 3'LTR gRNA and barcode

Plasmid

#127397

Purpose

Digestion by BstXI and BamHI to release the backbone

Depositor

Xiaowei Zhuang

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

Type

Empty backbone

Use

Lentiviral

Tags

Puro-mCherry

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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gS gag LV

Plasmid

#12327

Depositor

Inder Verma

Article

Kootstra et al Proc Natl Acad Sci U S A. 2003 Feb 4. 10

Insert

gag/pol

Use

Lentiviral

Expression

Mammalian

Mutation

The CypA binding region of the WT gag was replace…

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pGSTag DLC1

Plasmid

#24267

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1 Human)

Tags

Gst

Expression

Bacterial

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pMK17-02

Plasmid

#99604

Purpose

Plasmid containing parBp(mut)-m(sf)gfp fusion

Depositor

Morten Kjos, Jan-Willem Veening

Article

van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

Insert

parBp(mut)

Use

Synthetic Biology

Tags

m(sf)gfp

Mutation

internal parS-site mutated

Promoter

PczcD2

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 N-MLSN

Plasmid

#21082

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TRPM1 (TRPM1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pDRf1-SweetTrac1

Plasmid

#169083

Purpose

Expression of SweetTrac1 biosensor in yeast

Depositor

Lily S Cheung

Article

Park et al Proc Natl Acad Sci U S A. 2022 Jan 25;11

Insert

SweetTrac1

Expression

Yeast

Promoter

PMA1

Available Since

Feb. 4, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRSETA-alphaFlincG

Plasmid

#19735

Depositor

Wolfgang Dostmann

Article

Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

alpha-FlincG

Use

Protein expression

Tags

His

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pGP4G-TPLRD1-ccdB

Plasmid

#83877

Purpose

Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFThGW

Plasmid

#27228

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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LSSmKate1-C1

Plasmid

#54794

Purpose

Localization: C1 Cloning Vector, Excitation: 463, Emission: 624

Depositor

Michael Davidson, Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Type

Empty backbone

Tags

LSSmKate1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pg50-2.11 (#65)

Plasmid

#35966

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Insert

Tie2 promoter-1st exon-1st intron

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pHL661

Plasmid

#37635

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-H-RAN-RFP6

Plasmid

#106426

Purpose

H-RAN-RFP6 (RANbody RFP6::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-RFP6

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-PuroR_lenti

Plasmid

#138317

Purpose

Lenti expression of SpyCas9 with Puro selection

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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AlACR1_EYFP_pcDNA3.1

Plasmid

#161022

Purpose

Expresses strongly red-shifted AlACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

AlACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1 (M3)

Plasmid

#45896

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 M3 mutant (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

M3 (W229R) in SH3 domain

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-Nck1 (SH2 mut)

Plasmid

#45893

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 SH2 mutant (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

in SH2 domain (R308K) and E302K and Q333H

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only