We narrowed to 7,972 results for: lif

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eMagAF-EGFP-PM

Plasmid

#162247

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-CAAX

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBR-Lent-STAT5A-CA

Plasmid

#170662

Purpose

Lentiviral expression of constitutively active STAT5A.

Depositor

Alexander Ruthenburg

Article

Richter et al Elife. 2021 Jul 15;10. pii: 64960. doi:

Insert

STAT5A (STAT5A Human)

Use

Lentiviral

Mutation

H298R and S710F

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC7-syt1-GCaMP6f

Plasmid

#190896

Purpose

GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

Depositor

Tom Clandinin

Article

Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

Insert

syt1-GCaMP6f

Expression

Insect

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PTGS1-1

Plasmid

#184731

Purpose

COX1-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PTGS1-2

Plasmid

#184732

Purpose

COX1-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PTGS2-3

Plasmid

#184733

Purpose

COX2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-homo_PTGS2-4

Plasmid

#184734

Purpose

COX2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human COX2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Paxillin WT

Plasmid

#186152

Purpose

For expressing paxillin variants in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA2 TKIT

Plasmid

#169442

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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Non tagged Avidin

Plasmid

#214835

Purpose

This plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Non tagged Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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epB_UbC_CymRV5-nls-GFP_DEx2

Plasmid

#119906

Purpose

ePiggyBac mammalian expression plasmid for CymR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

CymRV5-nls-GFP

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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LeGo-Renilla-BLAST

Plasmid

#122276

Purpose

Lentiviral vector for stable expression of Renilla luciferase.

Depositor

Demián Cazalla

Article

Gorbea et al Elife. 2019 Sep 20;8. pii: 50530. doi: 1

Insert

Renilla luciferase

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pHFS:SEP-rGluA2 TKIT donor

Plasmid

#169440

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-Dre-T2A-TagRFP

Plasmid

#167968

Purpose

Transient expression of Dre and TagRFP

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

Dre recombinase

Tags

TagRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS5c-mCherry-AsLOV2*(543)

Plasmid

#202712

Purpose

Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Insert

mCherry-AsLOV2*(543)

Expression

Bacterial

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA2 TKIT donor

Plasmid

#169421

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pME-miniTurbo

Plasmid

#166567

Purpose

Multisite Gateway middle entry clone encoding miniTurbo. Parton lab clone JRQ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-MTS-jAspSnFR3-mRuby3

Plasmid

#203492

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Lys-eMagA-EGFP

Plasmid

#162246

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

p18-eMagA-EGFP

Expression

Mammalian

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Thy1.1-GFP

Plasmid

#131703

Purpose

encodes Thy1.1 followed by a P2A site and GFP for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CaNAR2

Plasmid

#64728

Purpose

FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Tags

Cerulean3 and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pQE-30-HyPer3

Plasmid

#42132

Purpose

Genetically encoded H2O2 probe for ratiometric and fluorescence lifetime imaging

Depositor

Vsevolod Belousov

Article

Bilan et al ACS Chem Biol. 2012 Dec 20.

Insert

HYPER 3

Tags

6His

Expression

Bacterial

Promoter

T5

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-cPLA2-mEGFP

Plasmid

#184718

Purpose

Exogenous cPLA2WT

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

cPLA2-mEGFP

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA1 TKIT

Plasmid

#169441

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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V5-TurboID-Mfn1

Plasmid

#195155

Purpose

mammalian expression of TurboID tagged Mfn1

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

TurboID

Tags

Mfn1

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pACS129

Plasmid

#204594

Purpose

Histone H3.1-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 (H3C3 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKL10-mRuby-iATPSnFR1.0 bb118

Plasmid

#158980

Purpose

Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mruby-iATPSNfR1.0

Tags

mruby3

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#102557

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN1 TKIT donor

Plasmid

#169427

Purpose

Donor DNA for TKIT knockin of SEP to NR1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1-Prig-3::tagBFP

Plasmid

#165598

Purpose

rig-3 promoter derive BFP expression in AVA head neurons.

Depositor

Andrew Leifer

Article

Hallinen et al Elife. 2021 Jul 29;10:e66135. doi: 10.75

Insert

Prig-3, tagBFP

Expression

Worm

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHFS:SEP-rGluA1 TKIT donor

Plasmid

#169438

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-TRE-dTomato-GCaMP6s

Plasmid

#99864

Purpose

expresses dTomato and GCaMP6s under control of the Tet-off system

Depositor

Venkatesh Murthy

Article

Wallace et al Elife. 2020 Mar 9;9. pii: 50531. doi: 10

Insert

dTomato, GCaMP6s

Promoter

Tet-responsive promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+Paxillin-mKate

Plasmid

#105974

Purpose

in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Paxillin

Tags

mKate

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-TurboID-NS

Plasmid

#166566

Purpose

Multisite Gateway middle entry clone encoding TurboID. Parton lab clone JRP

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

Multisite gateway entry clone

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
epB_CAG_TetRFlag-nls-GFPx2_DEx2

Plasmid

#119910

Purpose

ePiggyBac mammalian expression plasmid for TetR-GFP fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

TetRFlag-nls-GFPx2

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDN3.1(+)-CaNARi

Plasmid

#64848

Purpose

FRET-based calcineurin (CaN) activation biosensor.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean and Venus

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCZGY3163

Plasmid

#101248

Purpose

GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

gfp11::rps-18 (rps-18 Nematode, Synthetic)

Use

Gateway

Tags

GFP11

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
N-GFP-Dcp2

Plasmid

#163585

Purpose

express N-terminal GFP tagged Dcp2 FL at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

DCP2 promoter

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
BFP-Rab5CA (Q79L)

Plasmid

#205705

Purpose

Mammalian expression of BFP-tagged mutant Rab5CA

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

RAB5A (RAB5A Human)

Expression

Mammalian

Mutation

Q79L

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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