We narrowed to 50,136 results for: des.1

1. BB1_12_pFBA1-1

   Plasmid

   #98505

   Purpose

   BB1 with insert: promoter PFBA1-1

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   FBA1-1 promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB-1

   Plasmid

   #210387

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Tags

   Expression

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORTMAGE-1

   Plasmid

   #72680

   Purpose

   Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

   Depositor

   Csaba Pál

   Article

   Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

   Insert

   mutL E32K
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   lower strength RBS before mutL E32K allele than i…

   Promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pGEMHE:mTREK-1

   Plasmid

   #224743

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS44i8GH-1

   Plasmid

   #207525

   Purpose

   Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR

   Depositor

   Pablo Ivan Nikel

   Article

   Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

   Insert

   oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
   

   Use

   CRISPR; Pseudomonas vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3XHalo_GFP_TRF1#1

   Plasmid

   #164644

   Purpose

   tag TRF1 with GFP and Halo

   Depositor

   Michael Lampson, Huaiying Zhang

   Article

   Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1E-1

   Plasmid

   #21193

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek1 (Esr1, MAP2K1 Human, Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Mek1 R4F fused to ER*, constitutive active

   Promoter

   Available since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. sgp27#1

   Plasmid

   #102759

   Purpose

   Expresses sgRNA targeting mouse p27

   Depositor

   Jin Chen

   Article

   Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

   Insert

   p27 (Cdkn1b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. M2E-1

   Plasmid

   #21206

   Depositor

   Paul Khavari

   Article

   Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

   Insert

   Mek2 (Esr1, MAP2K2 Human, Mouse)
   

   Use

   Subclone vector

   Tags

   Expression

   Mutation

   deletion of residues EQQKKRLE, S222D S226D in Mek…

   Promoter

   Available since

   July 24, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pAP1791-1

    Plasmid

    #105246

    Purpose

    human eGFP with homology arms for RAB11A (recoded )

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

    Insert

    eGFP with homology arms for RAB11A (recoded *) (RAB11A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSPgRNA_BLK_E2-1

    Plasmid

    #124474

    Purpose

    For CRISPR-mediated epigenome editing of human BLK gene locus

    Depositor

    Iouri Chepelev

    Article

    Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

    Insert

    BLK_E2-1_gRNA (BLK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. shFGF2#1

    Plasmid

    #157667

    Purpose

    Tet inducible knockdown of FGF2

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. shAPI5#1

    Plasmid

    #157665

    Purpose

    Tet inducible knockdown of API5

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO_FOXA1_#1

    Plasmid

    #70095

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO_HOXB13_#1

    Plasmid

    #70093

    Purpose

    Expression of shRNA to human HOXB13, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. lentiCRISPRv2_RB1#1

    Plasmid

    #174150

    Purpose

    Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOGS539_haPD-1

    Plasmid

    #226716

    Purpose

    Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    High affinity PD-1 microbody
    

    Use

    Tags

    HA tag and Mating factor alpha secretion signal

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKHH030_sgCtrl#1

    Plasmid

    #174142

    Purpose

    Lentiviral vector expressing a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRv2_sgCtrl#1

    Plasmid

    #174148

    Purpose

    Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. plentiCRISPv2_FDX1-1

    Plasmid

    #184488

    Purpose

    Lentivirus all in one CRISPR vector targeting human FDX1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    FDX1 (FDX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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