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pAP2042-1

Plasmid

#105244

Purpose

mammalian eGFP with TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with extra-sequence (TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker::eGFP)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGBKT7_AvrSr62-1

Plasmid

#233531

Purpose

Expresses AvrSr62-1 in yeast

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

AvrSr62-1

Tags

GAL4 BD, Myc tag

Expression

Yeast

Promoter

ADH1

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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TFAP4_BHLH_5-1

Plasmid

#185054

Purpose

Deletion of BHLH domain of mouse TFAP4 in combination with TFAP4_BHLH_3-2

Depositor

Constance Ciaudo

Article

Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

Insert

TFAP4_5_1_gRNA (Tcfap4 Mouse)

Use

Lentiviral

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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BB1_12_pFBA1-1

Plasmid

#98505

Purpose

BB1 with insert: promoter PFBA1-1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

FBA1-1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Control-1

Plasmid

#204442

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

L243A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCB-1

Plasmid

#210387

Purpose

All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

Depositor

Gilles van Wezel

Article

Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

Inserts

dCas9
CymR
sgRNA cassette

Use

CRISPR; Integrative vector for use in streptomyces

Mutation

BsaI-flanked RFP cassette inserted in place of sp…

Promoter

SP11 and SP30

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-1

Plasmid

#72680

Purpose

Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

Depositor

Csaba Pál

Article

Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

Insert

mutL E32K

Tags

none

Expression

Bacterial

Mutation

lower strength RBS before mutL E32K allele than i…

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
pGEMHE:mTREK-1

Plasmid

#224743

Purpose

In vitro transcription for Oocyte expression of mTREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pS44i8GH-1

Plasmid

#207525

Purpose

Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR

Depositor

Pablo Ivan Nikel

Article

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;

Use

CRISPR; Pseudomonas vector

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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L1E-1

Plasmid

#21193

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Human, Mouse)

Use

Retroviral

Mutation

Mek1 R4F fused to ER*, constitutive active

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
sgp27#1

Plasmid

#102759

Purpose

Expresses sgRNA targeting mouse p27

Depositor

Jin Chen

Article

Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

Insert

p27 (Cdkn1b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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M2E-1

Plasmid

#21206

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek2 (Esr1, MAP2K2 Human, Mouse)

Use

Subclone vector

Mutation

deletion of residues EQQKKRLE, S222D S226D in Mek…

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pAP1791-1

Plasmid

#105246

Purpose

human eGFP with homology arms for RAB11A (recoded )

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

eGFP with homology arms for RAB11A (recoded *) (RAB11A Human)

Expression

Bacterial

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_BLK_E2-1

Plasmid

#124474

Purpose

For CRISPR-mediated epigenome editing of human BLK gene locus

Depositor

Iouri Chepelev

Article

Saint Just Ribeiro et al Front Genet. 2022 Sep 7;13:1008582. doi:

Insert

BLK_E2-1_gRNA (BLK Human)

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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shFGF2#1

Plasmid

#157667

Purpose

Tet inducible knockdown of FGF2

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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shAPI5#1

Plasmid

#157665

Purpose

Tet inducible knockdown of API5

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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