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We narrowed to 34,882 results for: cas

Showing: 4781 - 4800 of 34882 results

  1. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208942-plasmid-map-sequence-id-415667

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  4. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  8. 783-Rx-Dual: Combinatorial RfxCas13d gRNA and array cloning backbone

    Plasmid
    #228362
    Purpose
    mU6- and hU6-driven expression of dual RfxCas13d gRNAs and arrays for combinatorial RNA knockdown.
    Depositor
    Michael Boettcher
    Article
    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral; Rfxcas13d grna expression …
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mU6 and hU6
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    228362-plasmid-map-sequence-id-457033

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  10. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid
    #221385
    Purpose
    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene
    Depositor
    Junwei Shi
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    221385-plasmid-map-sequence-id-435944

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  11. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138143
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138143-plasmid-map-sequence-id-275400

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  18. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118162
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118162-plasmid-map-sequence-id-240427

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Showing: 4781 - 4800 of 34882 results