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We narrowed to 25,285 results for: crispr

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Showing: 4781 - 4800 of 25285 results

  2. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  5. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  8. HR-TERT-SV40-GFP

    Plasmid
    #71397
    Purpose
    Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.
    Depositor
    Thomas Cech
    Publication
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TERT (TERT Human)
    Tags
    SV-40 GFP insertion in TERT promotor
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71397-plasmid-map-sequence-id-128453

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  13. pTransposon.CAG.CasRX-P2A-Blast

    Plasmid
    #212965
    Purpose
    Episomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.
    Depositor
    Roland Rad
    Publication
    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CasRx
    Use
    Transposon
    Tags
    P2A-Blasticidin resistance
    Expression
    Mammalian
    Promoter
    CAGGS
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    212965-plasmid-map-sequence-id-422557

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  16. pCMV-PE2-P2A-BSD

    Plasmid
    #174819
    Purpose
    Mammalian expression of SpCas9 prime editor 2 with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2-P2A-BSD
    Tags
    P2A-BSD and SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174819-plasmid-map-sequence-id-343617

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  17. AICSDP-29: LMNB1-mTagRFP-T

    Plasmid
    #114403
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    114403-plasmid-map-sequence-id-220813

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  19. pCMV-PE-Plus

    Plasmid
    #214079
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn
    Depositor
    Ting Zhou
    Publication
    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE-Plus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214079-plasmid-map-sequence-id-429365

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Showing: 4781 - 4800 of 25285 results