We narrowed to 19,636 results for: tec

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pBJ5

Plasmid

#167134

Purpose

A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Frr promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTPH412

Plasmid

#185724

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-gp41-8N

Use

CRISPR

Expression

Bacterial

Mutation

ecTadA(8e) R26G

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSplit2-NO-PRPF6

Plasmid

#51740

Purpose

Express LucN-PRPF6 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

PRP6 (PRPF6 Human)

Tags

FLAG and Luciferase (1-398aa)

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pET-SisTnpB1–ωRNA

Plasmid

#228365

Purpose

Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

Depositor

Tao Liu

Article

Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

Insert

SisTnpB1

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-c-Jun

Plasmid

#171438

Purpose

Expression of HaloTag N58-(GGS)X6-c-Jun in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-c-Jun

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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UPB-stomach (UPB1)

Plasmid

#180784

Purpose

Unique Projection Barcode (UPB1)-Stomach for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

humanized ChR2-H134R (partial)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Cas9-NRCH

Plasmid

#136926

Purpose

Expresses Cas9-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

Cas9-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMR-A-3

Plasmid

#138517

Purpose

P3a

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_ATP1.0mut

Plasmid

#167578

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTK317

Plasmid

#59571

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTPH405c

Plasmid

#185726

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C) single PAM linker

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-10His-StayGold

Plasmid

#211362

Purpose

Mammalian expression of the bright and photostable StayGold fluorescent protein. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

StayGold

Tags

10xHis-tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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UPB-lung (UPB2)

Plasmid

#180788

Purpose

Unique Projection Barcode (UPB2)-Lung for Projection-seq

Depositor

Rui Chang

Article

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

humanized ChR2-H134R (partial)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I

Plasmid

#179685

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR

Expression

Mammalian

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB4906

Plasmid

#106150

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying hygromycin resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMM178

Plasmid

#61270

Purpose

Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

pLtetO, native, native (respectively)

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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HiFi-nCas9-NG_P2A-mCherry

Plasmid

#219936

Purpose

Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

HiFi-nCas9(D10A/R691A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_112

Plasmid

#136475

Purpose

Lentiviral expression of AsCas12a

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

AsCas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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EasyFusion Halo-mAID

Plasmid

#112852

Purpose

General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Halo-mAID

Use

Synthetic Biology

Promoter

No

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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nCas9-SpRY_P2A-mCherry

Plasmid

#219935

Purpose

Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-SpRY_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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