We narrowed to 10,059 results for: Uty

1. pKLV2-U6gRNA5(Vps39-g1)-PGKpuroBFP-W

   Plasmid

   #105019

   Purpose

   Lentiviral gRNA plasmid targeting mouse Vps39 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Vps39 (Vps39 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFUSEss-CHIg-mG3_Lys322Arg

   Plasmid

   #105862

   Purpose

   Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Lys322Arg (EU numbering).

   Depositor

   Joanna Bereta

   Article

   Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

   Insert

   IgG3 heavy chain (Ighg3 Mouse)
   

   Expression

   Mammalian

   Mutation

   Lys322Arg in constant region of IgG1 heavy chain

   Promoter

   hEF1-HTLV prom

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Hy_pMT Flag-UAP56 MUT

   Plasmid

   #110124

   Purpose

   Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   UAP56 (DDX39B Human)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   4 mutations (KSLN motif changed to RSFS)

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. ADAP1

   Plasmid

   #163905

   Purpose

   Full length mouse ADAP1 for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p28BIOHTEV-LIC: nsp16_SARS-CoV-2

   Plasmid

   #167846

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp16

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp16
   

   Tags

   6xHis and AviTag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAG150 FGFR1 3'UTR

   Plasmid

   #12008

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Insert

   N15 3'UTR (FGFR1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   June 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNAmyc-hPR-B-486-933

   Plasmid

   #89118

   Purpose

   mammalian expression of human progesterone receptor amino acid residues 486-933 with N-terminal myc tag, has PR DNA and ligand binding domains

   Depositor

   Elizabeth Wilson

   Article

   Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

   Insert

   human progesterone receptor ligand binding domain (PGR Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   contains aa 486-933

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCKO_PSMB2_sgRNA_5

   Plasmid

   #74185

   Purpose

   lentiviral vector expressing sgRNA targeting PSMB2

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   PSMB2 sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF Doc2beta 6X mutant GFP

   Plasmid

   #128823

   Purpose

   To visualize Doc2beta 6X mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D163A, D218A, D220N, D303A, D357A, D359A

   Promoter

   EF-1alpha

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. G protein alpha-o-R179C-EE

    Plasmid

    #55780

    Purpose

    This is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    G protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
    

    Tags

    EE epitope was introduced internally with D167E a…

    Expression

    Bacterial and Mammalian

    Mutation

    Arginine 179 was replaced with cysteine

    Promoter

    CMV

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYC-mTET1-N

    Plasmid

    #81056

    Purpose

    bacterial expression of N-terminus of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    N-terminus, amino acids 301-1366

    Promoter

    T7

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFREE019Env

    Plasmid

    #137670

    Purpose

    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)

    Depositor

    Anjali Joshi

    Article

    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.

    Insert

    HIV-1 Envelope Glycoprotein (env HIV-1)
    

    Expression

    Mammalian

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_TAP_FAM86A

    Plasmid

    #85116

    Purpose

    mammalian expression of FAM86A

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EEF2KMT (FAM86A Human)
    

    Tags

    CBP-SBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1925

    Plasmid

    #105869

    Purpose

    High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    iCreERT2
    

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTG-mTDGa.1

    Plasmid

    #81049

    Purpose

    bacterial expression of catalytically dead murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    asparagine 151 changed to alanine

    Promoter

    T7

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG75 ARHGEF18 3'UTR mut

    Plasmid

    #12055

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N1 3'UTR mut (ARHGEF18 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG175 AK024929 3'UTR mut

    Plasmid

    #12033

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N8 3'UTR mut (LINC00341 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUAST attB Orco (Y478A)

    Plasmid

    #73928

    Purpose

    UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    Orco (Y478A) (Orco Fly)
    

    Use

    Unspecified

    Mutation

    changed 478 Tyrosine for Alanine

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet-pLKO shMKP7.v1 puro

    Plasmid

    #87794

    Purpose

    Expresses an inducible short hairpin targeting human MKP7 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP7 (DUSP16 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG77 GLCCI1 3'UTR mut

    Plasmid

    #12062

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    GLCCI1 3'UTR mut (GLCCI1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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