166,697 results

1. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV_hSyn1-SIO-stGtACR2-FusionRed

   Plasmid

   #105677

   Purpose

   Expresses a soma-targeted GtACR2 in Cre-expressing neurons

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   stGtACR2
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRluc-LC3wt

   Plasmid

   #105002

   Purpose

   Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AICSDP-8:TOMM20-mEGFP

   Plasmid

   #87423

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAAV-CaMKIIa-EGFP

   Plasmid

   #50469

   Purpose

   EGFP under the control of CaMKIIa promoter

   Depositor

   Bryan Roth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHTN-HaloTag_BCL11A:1-773

   Plasmid

   #211014

   Purpose

   Mammalian expression of full-length human BCL11A. N-terminal HaloTag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   BCL11A:M1-F773 (BCL11A Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   isoform 2

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCIP-Flag-TfebAA

   Plasmid

   #79014

   Purpose

   For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Tfeb (Tfeb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S142A, S211A

   Promoter

   pCAGGs

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3/Flag-METTL3

   Plasmid

   #53739

   Purpose

   For expression of METTL3 (methyltransferase-like 3) in human cells

   Depositor

   Chuan He

   Article

   Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

   Insert

   Methyltransferase-like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C3_SEPT6

   Plasmid

   #71583

   Purpose

   mammalian expression of human SEPT6 with a GFP fusion

   Depositor

   Elias Spiliotis

   Article

   Hu et al Curr Biol. 2012 Jun 19;22(12):1109-15. d

   Insert

   Septin 6 (SEPTIN6 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   V206D

   Promoter

   CMV

   Available since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-DIO-tdTomato

    Plasmid

    #72254

    Purpose

    Express tdTomato under EF-1 promoter when Cre is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    tdTomato
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-EglN3-pcDNA3

    Plasmid

    #18960

    Depositor

    William Kaelin

    Article

    Lee et al Cancer Cell. 2005 Aug . 8(2):155-67.

    Insert

    Egg laying Nine - 3 (EGLN3 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 beta1

    Plasmid

    #16042

    Depositor

    Filippo Giancotti

    Article

    Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

    Insert

    beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDIO-DSE-mCherry-PSE-MCS

    Plasmid

    #129669

    Purpose

    Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

    Depositor

    Beatriz Rico

    Article

    Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

    Type

    Empty backbone

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 Rat, hybrid gene of rat and human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-Rab35 S22N inactive

    Plasmid

    #47426

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 S22N (RAB35 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S22N inactive

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR223-BLLF1(gp350)

    Plasmid

    #195837

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BLLF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BLLF1 (BLLF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1(+)-NEMOs

    Plasmid

    #189931

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZ M2rtTA_CAGG TetON-Sox10

    Plasmid

    #115240

    Purpose

    RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZmUBQ:LUC

    Plasmid

    #131860

    Purpose

    Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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