We narrowed to 6,286 results for: tTA

Showing: 4921 - 4940 of 6286 results

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AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-3

Plasmid

#180402

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-3 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-v2-ALDH1A3gRNA2

Plasmid

#189747

Purpose

The construct was used for expression of gRNA and Cas9 for knocking out of the ALDH1A3 gene.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

Cas9, ALDH1A3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_2 (inducible)

Plasmid

#189958

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.3 (H3f3b) sgRNA

Plasmid

#186931

Purpose

H3f3b sgRNA used for genomic targeting at H3f3b C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

H3f3b sgRNA (H3f3b Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK9c_2

Plasmid

#185023

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK9c

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_1

Plasmid

#185020

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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psiCheck2-SV40p-mtIF3(SR)-3xFLAG-HSVTKp-mCherry

Plasmid

#182381

Purpose

Dual expression construct encoding shRNA-resistant mtIF3 and mCherry from separate promoters

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Inserts

mtIF3 (Mtif3 Mouse)
mCherry

Tags

3xFlag

Expression

Mammalian

Mutation

Changed CDS sequence from 'ccacgttcaagtcacga…

Promoter

HSV TK promoter and SV40 promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_COX4

Plasmid

#177983

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting COX4

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting COX4

Use

Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.13.hSyn.H2B.RFP

Plasmid

#170372

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Bmpr2 gRNA#2

Plasmid

#163389

Purpose

Cas9-mediated knockout of Bmpr2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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Limk1 gRNA#2

Plasmid

#163392

Purpose

Cas9-mediated knockout of Limk1 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Limk1 (Limk1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh3

Plasmid

#168987

Purpose

Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9

Expression

Mammalian

Promoter

Ubc promoter

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-2

Plasmid

#83935

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCTRL-3

Plasmid

#83936

Purpose

Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCTRL-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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PX458-Lck

Plasmid

#159430

Purpose

CRISPR knockout of Lck

Depositor

Arthur Salomon

Article

Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

Insert

sgRNA targeting Lck exon 2 (LCK Human)

Use

CRISPR

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6, CBh

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-L

Plasmid

#122343

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ncapd3-R

Plasmid

#122330

Purpose

Expresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ncapd3

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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