We narrowed to 11,635 results for: Vars

1. pLKO.1P CISD1_2

   Plasmid

   #160774

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19_CoV-2_linker

   Plasmid

   #211731

   Purpose

   Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

   Depositor

   Thomas Klimkait

   Article

   Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

   Insert

   Linker fragment SARS-CoV-2
   

   Expression

   Mammalian

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-dCas12a-A3A

   Plasmid

   #193638

   Purpose

   Expression of base editor dCas12a-A3A in mammalian cells for base editing, along with non-fused EGFP fluorescence

   Depositor

   Liangxue Lai

   Article

   Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

   Insert

   dLbCas12a-hA3A, EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

   Plasmid

   #232244

   Purpose

   Mammalian expression of N581K RLIM-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   N581K RLIM-GFP-IRES-mCherry (RLIM Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   N581K

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

   Plasmid

   #232240

   Purpose

   Mammalian expression of REX1[degron]-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   REX1 (aa 70-85)

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS1995

   Plasmid

   #49125

   Purpose

   AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple94-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   GPR88

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCKC014

   Plasmid

   #226623

   Purpose

   pLenti wt hTdT template for library 3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   Terminal deoxynucleotidyl transferase (DNTT Human)
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Mutation

   codon optimized for human

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP1101

   Plasmid

   #65773

   Purpose

   Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, and T1337R mutations in C…

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_CTTN-1/1

    Plasmid

    #91550

    Purpose

    Protein expression and purification of human SH3 domain construct CTTN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CTTN-1/1 (CTTN Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPH2A.X_S139A-GS3

    Plasmid

    #204749

    Purpose

    Expression of Cas9 and human H2AX with the S139A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139A (H2AX Human)
    

    Expression

    Mammalian

    Mutation

    H2AX with the S139A mutation

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

    Plasmid

    #233035

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx
    

    Use

    AAV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEMS2232

    Plasmid

    #158401

    Purpose

    High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple316 (NR2E1 Human)
    

    Use

    Unspecified

    Promoter

    Ple316

    Available Since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 6xHis-MBP-BrCas12b

    Plasmid

    #182276

    Purpose

    Expresses recombinant BrCas12b

    Depositor

    Piyush Jain

    Article

    Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, T7, Thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFR D837A

    Plasmid

    #11014

    Purpose

    Retroviral construct for expressing human EGFR with D837A mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR D837A (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    D837A

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-RON-V5/HIS

    Plasmid

    #201983

    Purpose

    expression of human RON receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RON receptor tyrosine kinase (MST1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUG-natNT2

    Plasmid

    #110922

    Purpose

    expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    nat
    

    Expression

    Bacterial and Yeast

    Promoter

    AgTEF1

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBW_0065

    Plasmid

    #102817

    Purpose

    pVec8 containing Sr22 gene

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    wheat stem rust resistance gene Sr22 (NEWENTRY Synthetic)
    

    Expression

    Plant

    Promoter

    Native

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBW_0059

    Plasmid

    #102819

    Purpose

    pAGM4723 containing Sr35 gene

    Depositor

    Brande Wulff

    Article

    Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

    Insert

    wheat stem rust resistance gene Sr35 (NEWENTRY Synthetic)
    

    Use

    Synthetic Biology

    Promoter

    Native

    Available Since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CoV2-N-B.1.1.7

    Plasmid

    #177958

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    

    Expression

    Mammalian

    Mutation

    D3L,R203K,G204R,S235F

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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