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Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianMutationPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCP-YPet
Plasmid#215747PurposeExpresses MS2 coat protein (MCP) fused to YPetDepositorInsertYPet
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-po-mCerulean
Plasmid#213861PurposeBinary vector for the expression of peroxisome transit peptide fused mCerulean in plants (for Agrobacterium-mediated genetic transformation and visualization of peroxisome.)DepositorInsertPeroxisome transit peptide fused mCerulean
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher (Min)
Plasmid#216713PurposeExpresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014DepositorInsertCys-SpyCatcher (Min)
UseTags6xHisExpressionBacterialMutationSee Li...Howarth, J. Mol. Biol., 2014PromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH2.18_TagBFP
Plasmid#205533PurposeExpress TagBFPDepositorInsertTagBFP
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLipoP1-NLS-3xmCitrine
Plasmid#192522PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertPutative membrane lipoprotein (AT3G18250 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterLipoP1Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB UbC Arrestin-uTEV1-V5; UAS-mCherry
Plasmid#173122PurposeExpresses uTEV1 and reporter receiver construct in DIPGDepositorInsertArrestin-uTEV1-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-hLOV-TEVcs-Gal4
Plasmid#173120PurposeExpresses CCR6 hLOV receiver construct in DIPGDepositorInsertCCR6-hLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry
Plasmid#173121PurposeExpresses TEVp(WT) and reporter receiver construct in DIPGDepositorInsertArrestin-TEVp(WT)-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1
Plasmid#188975PurposeA human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.DepositorInsertg1 guide RNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1
Plasmid#188976PurposeA human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.DepositorInsertg2 guide RNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Plekhh1-FERM
Plasmid#187371PurposeExpresses human Plekhh1-FERM labelled with EGFPDepositorInsertPlekhh1-FERM
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Plekhh1-CTer
Plasmid#187369PurposeExpresses human Plekhh1-CTer labelled with EGFPDepositorInsertPlekhh1-CTer
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Plekhh1-MyTH-FERM
Plasmid#187370PurposeExpresses human Plekhh1-MyTH-FERM labelled with EGFPDepositorInsertPlekhh1-MyTH-FERM
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Plekhh1-NTer
Plasmid#187368PurposeExpresses human Plekhh1-NTer labelled with EGFPDepositorInsertPlekhh1-NTer
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1-IQ-Tail
Plasmid#187363PurposeExpresses rat myosin 1b isoform b IQTail domain labelled with EGFPDepositorInsertmyosin 1b isoform b IQTail domain (Myo1b Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
CcCDP_p15A
Plasmid#179272PurposeExpresses CcCDP in E. coli BL21(DE3)DepositorInsertcellodextrin phosphorylase
UseSynthetic BiologyTags6xHISExpressionBacterialMutationPromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Xalb_I-F1_array_GG
Plasmid#178770PurposeGolden Gate vector to generate X. albilineans type I-F1 single arraysDepositorInsertGolden Gate vector BaeI recognition site to generate X. albilineans type I-F1 single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sth_I-E array_GFP dropout
Plasmid#178775PurposeGolden Gate GFP dropout vector to generate S. thermophilus type I-E single arraysDepositorInsertgfp dropout to generate S. thermophilus type I-E single arrays
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEh_gRNA1
Plasmid#178758PurposeExpression of a gRNA that targets next to PAM library, used for E. haloalkaliphila type I-E systemDepositorInsertgRNA that targets next to PAM library, used for E. haloalkaliphila type I-E system
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only