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pWaldo-GFPd_PepTSt

Plasmid

#58333

Purpose

Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

Simon Newstead

Article

Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

Insert

PepTSt

Tags

GFP-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only
pPV477

Plasmid

#42930

Depositor

James Lok

Insert

attL1::egfp (870 bp)::attL2

Use

Multi-site gateway donor plasmid

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-6.22

Plasmid

#41087

Depositor

James Inglese

Article

Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

Insert

GSG-P2A-RLuc

Use

Luciferase

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 13, 2012

Availability

Academic Institutions and Nonprofits only
Pk-SOD1

Plasmid

#25574

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

Pk-SOD1

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pUS200

Plasmid

#17788

Depositor

Stephen Sligar

Article

Unger et al J Biol Chem. 1986 Jan 25. 261(3):1158-63

Insert

Cytochrome P450cam

Expression

Bacterial

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine (AAV8)

Viral Prep

#228902-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine (#228902). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine plasmid DNA. CAG-driven, Cre dependent expression of a peripherally restricted DREADD. These AAV preparations are suitable purity for injection into animals.

Depositor

Bryan Roth

Article

Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

Promoter

Chicken Beta Actin

Tags

mCitrine

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only
pAureus-TnCAM

Plasmid

#199807

Purpose

vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

Depositor

Steve Salipante

Article

Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

Insert

transposon cassette

Expression

Bacterial

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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JB28

Plasmid

#128900

Purpose

mammalian expression, proximity labelling, mitochondrial relocalisation

Depositor

Sean Munro

Article

Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

Insert

Rab5AQL-BirA*-HA-MAO (RAB5A Human)

Expression

Mammalian

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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T7_CC_Vpx-SIV_WPRE_IVT_Template

Plasmid

#216792

Purpose

Template for in vitro transcription of Vpx (SIV).

Depositor

Daniel Bauer

Article

Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

Insert

Vpx (SIV)

Expression

Mammalian

Promoter

T7

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDest-521

Plasmid

#159688

Purpose

Gateway destination vector for E. coli protein expression

Depositor

Dominic Esposito

Article

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v1

Plasmid

#113933

Purpose

Express an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFP

Depositor

Yubin Zhou

Article

Jing et al Nat Cell Biol. 2015 Oct;17(10):1339-47.

Insert

LOV2 and Rit polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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shRNA GR

Plasmid

#24095

Depositor

Moses Chao

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 1

Insert

Glucocorticoid Receptor (Nr3c1 Rat)

Use

Lentiviral

Tags

GFP

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pXGAD4eGFP

Plasmid

#25866

Depositor

Sergey Kasparov

Article

Wang et al FASEB J. 2006 Jul . 20(9):1537-9.

Insert

GAD4-EGFP

Expression

Mammalian

Available Since

Dec. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pGL2B -78/+64

Plasmid

#24947

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pMDC32B-AtTAS1c-D2-B/c-AtMIR173

Plasmid

#137885

Purpose

Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

Depositor

Alberto Carbonell

Article

Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

Inserts

AtTAS1c-D2-B/c
2x35S-AtMIR173-Tnos

Expression

Plant

Mutation

A. thaliana TAS1c precursor sequence including a …

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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pv6_CBX7_2xChromo

Plasmid

#179396

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

CBX7_2xChromo

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

G113V in flexible linker

Promoter

CAGGS

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pNIR-FbmCherry

Plasmid

#184676

Purpose

NIR-Fb to mCherry

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbmCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-FAF1

Plasmid

#107986

Purpose

expression of FAF1

Depositor

Georg Weber

Article

Weber et al Sarcoma. 2015;2015:839182. doi: 10.1155/

Insert

FAF1 (FAF1 Human)

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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