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pFETCh_MLX

Plasmid

#86277

Purpose

Donor vector for 3' FLAG tag of human MLX

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MLX homology arms (MLX Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMiniT:myc-GluA1 TKIT donor

Plasmid

#169419

Purpose

Donor DNA for TKIT knockin of Myc to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Myc (Myc Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2314

Plasmid

#36740

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCF-TALEN_Left (Fancf Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
pDGE7

Plasmid

#79462

Purpose

sgRNA shuttle vector; module 2

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE9

Plasmid

#79465

Purpose

sgRNA shuttle vector; module 3

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE11

Plasmid

#79467

Purpose

sgRNA shuttle vector; module 4E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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TAL2322

Plasmid

#36748

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO1-TALEN_Left (FOXO1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
TAL2268

Plasmid

#36705

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO3-TALEN_Left (Foxo3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2269

Plasmid

#36706

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FOXO3-TALEN_Right (Foxo3 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pMOD_A3910

Plasmid

#91054

Purpose

Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

dTaCas9-VPR

Use

CRISPR

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0201

Plasmid

#91002

Purpose

Module A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A nickase

Use

CRISPR

Mutation

D10A

Promoter

35S

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_TEAD3_2

Plasmid

#86338

Purpose

Encodes gRNA for 3' target of human TEAD3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TEAD3 (TEAD3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_TEAD3_1

Plasmid

#86337

Purpose

Encodes gRNA for 3' target of human TEAD3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TEAD3 (TEAD3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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GTL-g

Plasmid

#81099

Purpose

Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

sfGFP

Use

Cre/Lox

Tags

sfGFP

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GTT-r

Plasmid

#81111

Purpose

Gene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCherry

Use

Cre/Lox

Tags

mCherry

Expression

Yeast

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBGT

Plasmid

#62667

Purpose

Donor vector for iRFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

iRFP

Use

Cre/Lox

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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TAL2275

Plasmid

#36712

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Right (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2400

Plasmid

#36803

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC6-TALEN_Left (Hdac6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pQCi

Plasmid

#154345

Purpose

Self-Cutting and Integrating CRISPR backbone (px458 derivative with Cas9-intron, sgRNA, and bait integration sites)

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLLX8

Plasmid

#79838

Purpose

Contains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vector

Depositor

Cammie Lesser, Stephen Lory

Article

Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

Type

Empty backbone

Use

Contains yeast counter selection gene, cyh2 and b…

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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