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pET-mGinger1

Plasmid

#119784

Purpose

Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mGinger1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S14

Plasmid

#128596

Purpose

Expresses tagless 30S ribosomal subunit protein S14 in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S14 (rpsN E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

Plasmid

#162804

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI_IRES_Blasticidin

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_NoBody*_Intein_DD

Plasmid

#162813

Purpose

STELLA plasmid for amber suppression of C-terminally labelled NoBody

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

NoBody(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

NoBody(Opal69,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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hisSUMO-mZFP57

Plasmid

#87224

Purpose

Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

ZFP57 (Zfp57 Mouse)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

Plasmid

#162802

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI

Plasmid

#162801

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-stl

Plasmid

#51721

Purpose

conditional gene knockdowns in Trypanosoma brucei

Depositor

Arthur Gunzl

Article

Brandenburg et al EMBO J. 2007 Nov 28;26(23):4856-66. Epub

Type

Empty backbone

Use

Conditional gene knockdown in t. brucei

Promoter

T7 (2X)

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human APC-2,3 (1362–1540)

Plasmid

#132631

Purpose

expresses human APC-2,3 (1362–1540) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

Insert

APC-2,3 (1362–1540)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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mEGFP-EF(N)-30nmSAH

Plasmid

#61679

Purpose

GFP-labeled SAH scaffold with EF Hand attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-EF(N)-30nmSAH

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pTL359

Plasmid

#168420

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP41

Expression

Yeast

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pTL335

Plasmid

#168397

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP24

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTL336

Plasmid

#168396

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

OsaHIP34

Expression

Yeast

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S3-M129C

Plasmid

#132949

Purpose

Expresses tagless S3-M129C mutant ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

E.coli ribosomal protein S3 (rpsC E.coli)

Expression

Bacterial

Mutation

M129C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S15-T79C

Plasmid

#133048

Purpose

Expresses tagless S15-T79C mutant ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S15 E.coli ribosomal protein (rpsO E.coli)

Expression

Bacterial

Mutation

T79C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S19-T48C

Plasmid

#128597

Purpose

Expresses tagless S19-T48C mutant 30S ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S19 (rpsS E.coli)

Expression

Bacterial

Mutation

T48C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTL95

Plasmid

#168329

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

Si514802025

Expression

Yeast

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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