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myc-Rictor

Plasmid

#1860

Purpose

Please see Addgene item #11367 for an updated version of this plasmid.

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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anti-FLAG M2 light chain

Plasmid

#175358

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 light chain

Depositor

Joost Snijder

Publication

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 light chain

Tags

N-terminal secretion signal peptide (MEAPAQLLFLLL…

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSP1

Plasmid

#20060

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of deletion mutant (1-43) of human…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Publication

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28a-SnoopCatcher

Plasmid

#72322

Purpose

Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

Depositor

Mark Howarth

Publication

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a-SnoopCatcher

Tags

His6 tag

Expression

Bacterial

Mutation

changed G842T and D848G of S. pneumoniae adhesin …

Promoter

T7

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Publication

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor Q63L (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Publication

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor T19N (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET-6xHis-(pos36)GFP

Plasmid

#62937

Purpose

Expression of 6xHis-(+36)GFP in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(pos36)GFP

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28-mE1

Plasmid

#32534

Depositor

Jorge Eduardo Azevedo

Publication

Carvalho et al Mol Biotechnol. 2011 Oct 20.

Insert

E1, ubiquitin-activating enzyme (Uba1 Mouse)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pAd-Track Flag-SIRT1

Plasmid

#8438

Depositor

Pere Puigserver

Publication

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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1318 pcDNA3 flag FKHRL1

Plasmid

#10708

Depositor

William Sellers

Publication

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKHRL1 (FOXO3 Human)

Tags

flag

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3/Myc-DNMT3L

Plasmid

#35523

Depositor

Arthur Riggs

Publication

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DNMT3L (DNMT3L Human)

Tags

Myc

Expression

Mammalian

Available since

March 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRZeroT

Plasmid

#120276

Purpose

Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Publication

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Publication

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pet30b-5M SrtA

Plasmid

#51140

Purpose

expression plasmid for C-terminal his tagged S. aureus SrtA with enhanced catalytic activity

Depositor

Hidde Ploegh

Publication

Shi et al Proc Natl Acad Sci U S A. 2014 Jul 15;11

Insert

S.aureus SrtA 5M

Tags

6x His

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis, TEV cleavage site, and TrxA

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Publication

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMSP1E1

Plasmid

#20062

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeat of hel…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET-dCas9-VP64-6xHis

Plasmid

#62935

Purpose

Expression of dCas9-VP64-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

dCas9-VP64

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A and H840A amino acid changes render Cas9 nuc…

Promoter

T7

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Publication

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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