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LeGO-iC2

Plasmid

#27345

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-mCherry

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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MI-Luciferase-IRES-mCherry

Plasmid

#75020

Purpose

Encodes the expression of Luciferase and mCherry

Depositor

Xiaoping Sun

Publication

Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

Inserts

firefly Luciferase
mCherry

Use

Luciferase and Retroviral

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLV_TRET_hNgn2_UBC_Puro

Plasmid

#61474

Purpose

3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection marker

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (NEUROG2 Human)
Puro

Use

Lentiviral

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLCNICK

Plasmid

#84653

Purpose

Genome editing for Lactobacillus casei Lc2W

Depositor

Sheng Yang

Publication

Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

Inserts

Cas9 nickase
sgRNA
homology arms of LC2W_2179

Use

CRISPR

Mutation

D10A

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAVS1-TLR targeting vector

Plasmid

#64215

Purpose

Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

Depositor

Ralf Kuehn

Publication

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

traffic light reporter (TLR) (AAVS1 Human)

Use

Human targeting

Mutation

see publication for details

Promoter

CAG

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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YAP/TAZ-TRE-mStrawberry reporter

Plasmid

#158682

Purpose

This plasmid is a YAP/TAZ pathway reporter. It has a YAP/TAZ-responsive synthetic promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Lenti-entry-puro

Plasmid

#85745

Purpose

Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

Depositor

Qin Yan

Publication

Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDG459

Plasmid

#100901

Purpose

SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

Depositor

Paul Thomas

Publication

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-PuroR

Expression

Mammalian

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAC93-pmax-dCas9VP160

Plasmid

#48225

Purpose

dCas9VP160 on pmax expression vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR

Tags

HA Tag and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

Plasmid

#138610

Purpose

Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

Depositor

Fei Chen

Publication

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

Hyperactive AID
T7 RNA Polymerase
tdTomato

Expression

Mammalian

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMK291 (mAID-mClover-Bsr)

Plasmid

#72829

Purpose

C-terminal tagging with mAID-mClover using CRISPR/Cas9

Depositor

Masato Kanemaki

Publication

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-mClover-Bsr

Use

CRISPR

Tags

mAID-mClover

Expression

Mammalian

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ152

Plasmid

#69303

Purpose

Gateway entry vector with pco-dCas9-VP64

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pco-dCas9-VP64 (Plant codon-optimized)

Use

CRISPR

Mutation

VP64 activator fusion; NLS fusion

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAC-LYC

Plasmid

#53270

Purpose

Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli

Depositor

Francis X Cunningham Jr

Publication

Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

Insert

crtE, crtI, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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LeGO-iT2

Plasmid

#27343

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-tdTomato

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#190163

Purpose

Microglia-targeted transgene expression.

Depositor

Hirokazu Hirai

Publication

Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p

Use

AAV

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Publication

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pJYS3_ΔcrtYf

Plasmid

#85542

Purpose

Constitutive transcription of FnCpf1 and crRNA of crtYf

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

Plasmid

#20944

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Publication

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

H134R

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
pDD284

Plasmid

#66825

Purpose

TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

TagRFP-T-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized TagRFP-T

Expression

Worm

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles