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pET-Neq2X7

Plasmid

#182366

Purpose

Expresses Neq2X7 DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X7 DNA polymerase

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pEcCas5

Plasmid

#170090

Purpose

expresses E. coli type I-E Cas5

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas5

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas6

Plasmid

#170091

Purpose

expresses E. coli type I-E Cas6

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas7

Plasmid

#170092

Purpose

expresses E. coli type I-E Cas7

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas7

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas8

Plasmid

#170093

Purpose

expresses E. coli type I-E Cas8

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCse2

Plasmid

#170094

Purpose

expresses E. coli type I-E Cse2

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cse2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

Plasmid

#80840

Purpose

Modified helper phage

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene II
M13 gene X
M13 gene V
M13 gene VII
M13 gene IX
M13 gene VIII
M13 gene I
M13 gene XI
M13 gene IV

Expression

Bacterial

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro

Plasmid

#216666

Purpose

Expresses tTa-inducible MyoD and miRFP703, with puromycin resistance

Depositor

Leonardo Morsut

Inserts

MyoD (Myod1 Mouse)
miRFP703
PuroR

Use

Lentiviral

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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hROSA26 CRISPR-pX330

Plasmid

#105927

Purpose

hROSA26 targeting CRISPR plasmid

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

hROSA26 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.E227

Plasmid

#138249

Purpose

Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

mCherry
Terminator
mTagBFP
Terminator
gfp
Terminator

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

P17_up1691, P20_992, and iP16_3622

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-eSIBR

Plasmid

#71664

Purpose

EGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backbone

Depositor

Philip Washbourne

Article

Fowler et al Nucleic Acids Res. 2015 Nov 17. pii: gkv

Insert

EGFP coding sequence followed by eSIBR cassette

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pXY027

Plasmid

#98915

Purpose

Express FtsZ tagged with GFP in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Tags

GFP

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pDA010.EL222

Plasmid

#206801

Purpose

J23106.EL222

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

EL222

Promoter

J23106

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII

Plasmid

#80863

Purpose

Phagemid (encodes evolved cI4A5T6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR eGFPCreERT2 L1-R5

Plasmid

#43961

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

eGFP-CreERT2

Use

Multisite gateway entr clone

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pDA040.BLD7-mRFP

Plasmid

#206800

Purpose

EL222_D7_mRFP

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

BLD7.mRFP

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-ETN-lox2272

Plasmid

#154833

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

ETN

Use

Cre/Lox

Expression

Mammalian

Promoter

100RPU.2

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pXalb_IC_Cascade

Plasmid

#178765

Purpose

Expression of X. albilineans type I-C Cascade genes

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

X. albilineans type I-C Cascade genes

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJethlpAmScarletCm

Plasmid

#206818

Purpose

Template for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mScarlet

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAYFPCm

Plasmid

#206826

Purpose

Template for hlpA-YFP amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

YFP

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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