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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,902 results for: multi

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Showing: 481 - 500 of 18902 results

  5. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Publication
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    122650-plasmid-map-sequence-id-437776

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  8. SepRS(2)/pSertRNA(B4)/EF-Sep

    Plasmid
    #173897
    Purpose
    The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.
    Depositor
    Jason W Chin
    Publication
    Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d
    Inserts
    SepRS(2)
    pSertRNA
    EF-Sep
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    173897-plasmid-map-sequence-id-342748

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  12. pCMV-lyso-pHoenix

    Plasmid
    #70112
    Purpose
    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes
    Depositor
    Christian Rosenmund, Benjamin Rost
    Publication
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)
    Tags
    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70112-plasmid-map-sequence-id-418643

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  18. PLJR965

    Plasmid
    #115163
    Purpose
    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Sarah Fortune
    Publication
    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    Use
    Unspecified
    Available since
    Sept. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115163-plasmid-map-sequence-id-222098

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  20. HIP-flash (HOP-flash mutant)

    Plasmid
    #83466
    Purpose
    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene
    Depositor
    Barry Gumbiner
    Publication
    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    Use
    Luciferase
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83466-plasmid-map-sequence-id-159299

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Showing: 481 - 500 of 18902 results