We narrowed to 19,001 results for: Tec

1. pFPL-Cg

   Plasmid

   #61652

   Purpose

   Gateway vector for generating cyan fluorescent protein fusions in fungi

   Depositor

   Mark Farman

   Article

   Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

   Insert

   enhanced cyan fluorescent protein
   

   Expression

   Plant

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTS2356_Tier3(PB)-PuroR

   Plasmid

   #169655

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH

   Plasmid

   #56659

   Purpose

   Dual-expression vector for P. tricornutum

   Depositor

   Philip Weyman

   Article

   Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

   Insert

   KanR
   

   Use

   Synthetic Biology; Diatom expression

   Available Since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM585-Tier1-PhCMV-Igk

   Plasmid

   #169486

   Purpose

   Tier-1 vector encoding PhCMV-driven secretion signal Igk (PhCMV-Igk-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Igk secretion signal (IGK Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEarleyGate103 mmPing20F

   Plasmid

   #140007

   Purpose

   mmPing20F activation tag

   Depositor

   Charles Nathan Hancock

   Article

   Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

   Insert

   mmPing20F
   

   Expression

   Plant

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-LbCas12a

   Plasmid

   #213743

   Purpose

   For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPC-Flag-TRF2(delta)iDDR

   Plasmid

   #44577

   Depositor

   Eros Lazzerini Denchi

   Article

   Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

   Insert

   TRF2ΔiDDR (TERF2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   iDDR domain (aa 407-431) has been deleted

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

8. EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)

   Plasmid

   #191179

   Purpose

   Negative control sensor in human cells.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s2:EGFP (negative control)
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G03 RUVBL1

   Plasmid

   #67091

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RUVBL1 (RUVBL1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PibpA GFP pZa

    Plasmid

    #170076

    Purpose

    GFP expression under the control of E. coli ibpA promoter

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    PibpA

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PfxsA GFP pZa

    Plasmid

    #170075

    Purpose

    GFP expression under the control of E. coli fxsA promoter

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Promoter

    PfxsA

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. YPRCΔ15α1

    Plasmid

    #101712

    Purpose

    GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 IL2

    Plasmid

    #67053

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IL2 (IL2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pOCC114

    Plasmid

    #118904

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with TEV protease, two glycines

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. CP1300-ABEmax

    Plasmid

    #119810

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

    Plasmid

    #154303

    Purpose

    Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Inserts

    histone H1
    γ-tubulin
    

    Use

    Dictyostelium expression

    Tags

    mEGFP and mRuby3

    Promoter

    actin15

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAL2385

    Plasmid

    #36794

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BRCA1-TALEN_Right (Brca1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pML3009

    Plasmid

    #193256

    Purpose

    Gamma-aminobutyric acid sensor expressing eYFP reporter

    Depositor

    Lauren Andrews

    Article

    Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

    Inserts

    gabR
    eYFP
    

    Use

    Synthetic Biology

    Promoter

    synthetic promoter P2 and synthetic promoter PGab…

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-hPDIb′a′

    Plasmid

    #60556

    Purpose

    enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

    Depositor

    Han Choe

    Article

    Song et al Appl Biochem Biotechnol. 2013 May;170(1)

    Type

    Empty backbone

    Tags

    His6-hPDIb'a' tag

    Expression

    Bacterial

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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