We narrowed to 80,363 results for: TRI

1. pJK385

   Plasmid

   #71572

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS1422

   Plasmid

   #63793

   Purpose

   Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Twin-Strep tag followed by URA3 selection marker

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Rai et al Mol Biotechnol. 2014 Jun 27.

   Insert

   eCitrine-Twin-tag followed by URA3 selection marker
   

   Use

   E. coli cloning vector

   Tags

   eCitrine-Twin-tag

   Expression

   Mutation

   Promoter

   none

   Available since

   April 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Xl-VSP1 R152Q

   Plasmid

   #51881

   Purpose

   expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available since

   May 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-myc-Rem2 S69A/S241A/S308A/S334A

   Plasmid

   #51594

   Purpose

   expresses RNAiR myc-tagged Rem2 S-A mutant

   Depositor

   Suzanne Paradis

   Article

   Ghiretti et al J Neurosci. 2013 Apr 10;33(15):6504-15.

   Insert

   Rem2 (Rem2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Serines 69, 241, 308, 334 to Alanines; also RNAi …

   Promoter

   CMV

   Available since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pVRc33_423

   Plasmid

   #49749

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   AS33_423
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pLux

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-puro-mMCL1 Delta C22

   Plasmid

   #45820

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   mMCL-1 Delta C22
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C-terminal 22 amino acids are deleted from full l…

   Promoter

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pMSCV-puro-Delta N67-mMCL1

   Plasmid

   #45819

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   Delta N67-mMCL-1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-terminus 67 amino acids deleted from full lengt…

   Promoter

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pFloxin-Ofd1-Myc-KDD359-361FSY

   Plasmid

   #24578

   Depositor

   Jeremy Reiter

   Article

   Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

   Insert

   Ofd1 (Ofd1 Mouse)
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Myc

   Expression

   Mutation

   Lysine-Aspartate-Aspartate 359-361 changed to Phe…

   Promoter

   Available since

   May 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCW-tTRE-scFv-SunTag

   Plasmid

   #193138

   Purpose

   Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   scFv-SunTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-GEM-GECO1

    Plasmid

    #32442

    Purpose

    Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGM6

    Plasmid

    #110660

    Purpose

    Adeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.

    Depositor

    David Russell

    Article

    Gregorevic et al Nat Med. 2004 Aug;10(8):828-34. doi: 10.

    Insert

    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pST_116_LVL2 cam

    Plasmid

    #179332

    Purpose

    NT-CRISPR plasmid for a single gRNA.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-myc-HLA-A2

    Plasmid

    #135504

    Purpose

    Mammalian expression of myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-mScarlet

    Plasmid

    #131001

    Purpose

    AAV vector to drive the expression of mScarlet under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV8

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mRFP-Ub

    Plasmid

    #11935

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin (RBX1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ntn4-Fc-His

    Plasmid

    #72106

    Purpose

    Expresses the entire Netrin 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntn4 (Ntn4 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-Ub KO

    Plasmid

    #11934

    Depositor

    Nico Dantuma

    Article

    Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

    Insert

    Ubiquitin KO (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    All 7 lysines of ubiquitin mutated to arginines.

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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