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pBIG2abcde

Plasmid

#80619

Purpose

pBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Neo GFP-Progerin C661S

Plasmid

#118711

Purpose

Lentiviral TET-ON inducible GFP-Progerin C661S (Farnesylation mutant)

Depositor

Tom Misteli

Article

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin C661S (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Mutation

C661S farnesylation mutant

Promoter

CMV TRE3G (TET-ON)

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSD080A

Plasmid

#218138

Purpose

Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD40-eGFP-IRES2-mCherry

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pST50Trc1-HISNDHFR

Plasmid

#63940

Purpose

Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Tags

6xHis; TEV cleavable

Expression

Bacterial

Promoter

T7

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGGB003

Plasmid

#48821

Purpose

Provides a default dummy as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

B-Dummy

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ166

Plasmid

#109328

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Insert

zCas9

Use

CRISPR; Gateway compatible zcas9 entry clone

Tags

3x FLAG, NLS and NLS

Expression

Plant

Mutation

Zea mays codon-optimized Cas9

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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Flag WDR24 pLJM1

Plasmid

#46337

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Use

Lentiviral

Tags

FLag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-YL1

Plasmid

#15333

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

transcription factor-like 1 (VPS72 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
SARS CoV-2 HexaPro spike (bio-His)

Plasmid

#166856

Purpose

Human expression vector encoding full length SARS CoV-2 spike protein, containing biotinylation site and 8His-tag. Modification of Addgene plasmid #154754.

Depositor

Evelien Bunnik

Article

Reyes et al PLoS One. 2021 Dec 22;16(12):e0261656. d

Insert

SARS CoV-2 HexaPro full length spike protein (S Severe acute respiratory syndrome-related coronavirus)

Tags

8xHis and Biotinylation site

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLV6-mPER2-luc

Plasmid

#212035

Purpose

Expression of murine Per2-luciferase in mammalian cells

Depositor

David Hazlerigg

Article

Appenroth et al J Biol Rhythms. 2024 Dec;39(6):554-567.

Insert

mPer2-luciferase (Per2 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-mitoT

Plasmid

#174519

Purpose

Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

Depositor

Oleh Khalimonchuk

Article

Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

Insert

MitoT synthetic tether

Tags

6xHis and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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DUSP6

Plasmid

#40772

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

DUSP6 (DUSP6 Human)

Tags

6His and TEV

Expression

Bacterial

Mutation

Starting Met removed

Promoter

T7

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-mBAF45b IRES GFP

Plasmid

#17881

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45b (Dpf1 Mouse)

Tags

FLAG and IRES EGFP

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMi(3xP3-DsRed)

Plasmid

#102539

Purpose

Transgenesis vector based on the Minos transposon, carrying the 3xP3-DsRed marker

Depositor

Michalis Averof

Article

Pavlopoulos et al Proc Natl Acad Sci U S A. 2005 May 31;10

Insert

Minos transpoable element with 3xP3-DsRed marker

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

Plasmid

#171384

Purpose

Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized dCas13X.1-RESCUE-S

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA2

Plasmid

#19485

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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MCP-ADAR2-DD(E488Q)

Plasmid

#170125

Purpose

Lentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MCP-ADAR2-DD(E488Q)-NES

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a core promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-PAmKate

Plasmid

#32691

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Expression

Bacterial

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

araB

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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