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pEGFP Cep170 C-term (Nigg GG2)

Plasmid

#41151

Depositor

Erich Nigg

Article

Guarguaglini et al Mol Biol Cell. 2005 Mar;16(3):1095-107.

Insert

Cep170 (CEP170 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains C-terminal fragment (aa 755-1460, from X…

Promoter

CMV, T7

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pTagGFP2-N SIN1 NCD

Plasmid

#124927

Purpose

For expression of NCD (a.a. 1-139) of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594A

Plasmid

#131717

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594A

Promoter

CMV

Available Since

Nov. 11, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-G469A

Plasmid

#131711

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

G469A

Promoter

CMV

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594N

Plasmid

#131713

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594N

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107010

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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AcGFP-MLH1

Plasmid

#215120

Purpose

Expresses AcGFP-MLH1 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

MLH1 (MLH1 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86022

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain; Changed alanine 82 to va…

Promoter

CMV IE

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-H-Ras(G12V)-IRES-Puro

Plasmid

#120560

Purpose

Expresses HA-birA*-H-Ras G12V mutant fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

HRAS (HRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX2

Plasmid

#206388

Purpose

Expresses human SOX2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX2 (SOX2 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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RNF138_pLX307

Plasmid

#98368

Purpose

Lentiviral expression of RNF138

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RNF138 (RNF138 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

Plasmid

#178827

Purpose

Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

SomQuasAr6a_EGFP-P2A-somCheRiff_HA

Use

AAV, Cre/Lox, and Mouse Targeting

Tags

EGFP, HA

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CCND2 targeting gRNA

Plasmid

#215318

Purpose

Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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CCND1 targeting gRNA

Plasmid

#215153

Purpose

Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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FokI(inactive)-mCherry-LacI-NLS

Plasmid

#215100

Purpose

Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FokI(inactive)-mCherry-LacI-NLS

Expression

Mammalian

Mutation

FokI(inactive) mutant D69A.

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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PRKCDPB_pLX307

Plasmid

#98359

Purpose

Lentiviral expression of PRKCDPB

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

PRKCDPB (CAVIN3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R8P

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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tdEos-Mito-7

Plasmid

#57644

Purpose

Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Mito (COX8A Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Mutation

Codon optimized C-terminal 230 amino acids

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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mcry1K31R HA tag

Plasmid

#110297

Purpose

mammalian expression of full length mouse CRY1 mutant K31R

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mcry1 K31R (Cry1 Mouse)

Tags

HA tag

Expression

Mammalian

Mutation

31 Lysines are changed to Argenine

Promoter

CMV

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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phBG-P53DD

Plasmid

#149707

Purpose

In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

Depositor

Erika Sasaki

Article

Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

Insert

Trp53 (Trp53 Mouse)

Use

Rna transcription

Mutation

deleted amino acid 14-301

Promoter

T7

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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