164,868 results

1. pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

   Plasmid

   #62949

   Purpose

   Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-LexA::QFAD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. DH10B-M.Osp807II

   Bacterial Strain

   #114267

   Purpose

   The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.

   Depositor

   Christopher A. O'Callaghan

   Article

   Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

   Bacterial Resistance

   Bleocin (zeocin)

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

3. pHAGE-EF1aL-nlsGFP-W

   Plasmid

   #126688

   Purpose

   Constitutive, ubiquitous, nuclear-localized GFP expression.

   Depositor

   Darrell Kotton

   Article

   Tang et al Nat. Phys. 18, 1371–1378 (2022).

   Insert

   nlsGFP
   

   Use

   Lentiviral

   Tags

   6xHIS and NLS

   Expression

   Mutation

   Promoter

   EF1aL

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRE4-Azi

   Plasmid

   #105829

   Purpose

   Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

   Depositor

   Irene Coin

   Article

   Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

   Inserts

   Humanized EAziRS
   4 copies of the U6-BstYam tRNA cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (Y37L, D182S, F183M, L186A, D265R)TyrRS

   Promoter

   PGK and U6

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCWori cytochrome P450-BM3h 9D7

   Plasmid

   #61308

   Purpose

   Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

   Depositor

   Alan Jasanoff

   Article

   Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

   Insert

   Cytochrome P450-BM3 heme domain 9D7
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T268A, I263A, T438V, A328G

   Promoter

   Tac

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Talin-TS

   Plasmid

   #83376

   Purpose

   FRET probe for measuring tension on Talin molecule

   Depositor

   Martin Schwartz

   Article

   Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

   Insert

   Talin1 (Tln1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-F40-tagRFP (tension sensor module)

   Expression

   Mammalian

   Mutation

   Tension sensor module is inserted between V447 an…

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG

   Plasmid

   #129632

   Purpose

   Visualization of Akt and ERK activity

   Depositor

   Dorus Gadella

   Article

   Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

   Insert

   PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIS3p:CloverGFP-Tub1+3'UTR::URA3

   Plasmid

   #50636

   Depositor

   Wei-Lih Lee

   Article

   Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

   Insert

   CloverGFP-Tub1
   

   Use

   Yeast expression

   Tags

   CloverGFP

   Expression

   Mutation

   Promoter

   HIS3p

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. tdTomato-H2B-6

   Plasmid

   #58101

   Purpose

   Localization: Nucleus/Histones, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRK-ATF4

    Plasmid

    #26114

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF4 (ATF4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-M1 Spastin

    Plasmid

    #134461

    Purpose

    M1 Spastin expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Met to Ala change at amino acid 87

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-WT-STAT1

    Plasmid

    #71454

    Purpose

    Lentiviral expression of WT-STAT1

    Depositor

    George Stark

    Article

    Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-G-Flamp2-mut

    Plasmid

    #192783

    Purpose

    control probe of G-Flamp2, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Liu et al Front Pharmacol. 2022 May 12;13:902290.

    Insert

    G-Flamp2-mut
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_BiPVe4_eGFP

    Plasmid

    #221463

    Purpose

    AAV construct expressing eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. IL6R PLAK1

    Plasmid

    #161518

    Purpose

    Expresses IL6 receptor (full length) in mammalian cells.

    Depositor

    Yoav Shaul

    Article

    Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

    Insert

    Interleukin 6 Receptor (IL6R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV5-Flag-RhoA wt

    Plasmid

    #11750

    Depositor

    Jeff Wrana

    Article

    Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pBTBX-1

    Plasmid

    #26067

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    hSyn

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMU-PERr

    Plasmid

    #61202

    Purpose

    Fluorescent reporter for peroxisomes mCherry-SKL expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    mCherry-SKL
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139093

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dxCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MGAT-SEP-mRuby3

    Plasmid

    #200937

    Purpose

    cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    MGAT2 (MGAT2 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

    Plasmid

    #86827

    Purpose

    Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    FLAG-NLS-MS2-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA3-Flag-IDH1

    Plasmid

    #62906

    Purpose

    Expresses wildtype IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSP6-RNAP

    Plasmid

    #48143

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    rna polymerase SP6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    V314A (numbering based on NCBI Sequence ID: NP_85…

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLX304 ERG

    Plasmid

    #118625

    Purpose

    Overexpresses ERG protein

    Depositor

    William Hahn

    Article

    Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

    Insert

    ERG (33-479) (ERG Human)
    

    Use

    Lentiviral

    Tags

    None and none

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gTBEv5

    Plasmid

    #220621

    Purpose

    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

    Insert

    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

    Promoter

    hU6, EF1a

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Rictor_2 shRNA

    Plasmid

    #1854

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Rictor (RICTOR Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-hRedOpsin-TGFB1

    Plasmid

    #164427

    Purpose

    AAV plasmid expressing TGFB1 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

    Insert

    Tgfb1 (Tgfb1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human red opsin

    Available since

    Nov. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBabe puro c-met WT

    Plasmid

    #17493

    Depositor

    Joan Brugge

    Article

    Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

    Insert

    c-Met (Met Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Mito-SBFP2

    Plasmid

    #187964

    Purpose

    Mammalian cell expression of a fluorescent SBFP2-tagged mitochondrial marker

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    SBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Anti-Integrin alpha V beta 3 [IPI-ITGAV/ITGB3.7] (Recombinant Antibody)

    Antibody

    #213660-rAb

    Purpose

    Anti-Integrin αVβ3 (Human) chimeric recombinant antibody with fused human variable and rabbit constant domains; binds the particular alpha/beta chain pair, blocks ligand binding/function (RGD-mimetic)

    Depositor

    Institute for Protein Innovation (IPI)

    Article

    Jo et al Nat Commun. 2022 Dec 3;13(1):7471. doi:

    Recommended Applications

    Flow Cytometry

    Reactivity

    Human and Mouse

    Source Species

    Rabbit

    Isotype

    IgG1

    Trial Size

    Not available to purchase

    Available since

    March 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

35. pHIS3p:CloverGFP-Tub1+3'UTR::HIS3

    Plasmid

    #50654

    Depositor

    Wei-Lih Lee

    Article

    Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

    Insert

    CloverGFP-Tub1
    

    Use

    Yeast expression

    Tags

    CloverGFP

    Expression

    Mutation

    Promoter

    HIS3p

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AMA1-bio

    Plasmid

    #47741

    Purpose

    Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised AMA1
    

    Use

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TFORF1977

    Plasmid

    #144120

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TBX1 (TBX1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MiCy-pBAD

    Plasmid

    #54565

    Purpose

    Localization: Protein Expression Vector , Excitation: 472, Emission: 495

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

    Type

    Empty backbone

    Use

    Tags

    6xHis and MiCy

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only