We narrowed to 23,148 results for: REP

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pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

Plasmid

#162335

Purpose

Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pPN440

Plasmid

#137874

Purpose

PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPN458

Plasmid

#137876

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNFGFR1-HA

Plasmid

#130887

Purpose

Expresses dominant negative mutant mouse FGFR1

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

Fgfr1 (Fgfr1 Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pACEC3GH (Apr)

Plasmid

#110085

Purpose

Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW-ZipACR-EYFP

Plasmid

#88844

Purpose

Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

ZipACR-NotI-EYFP

Use

Lentiviral

Tags

EYFP

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70

Plasmid

#62946

Purpose

Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-QF2-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCI5-CasRx-HA (pKW88)

Plasmid

#229046

Purpose

Mammalian expression of CasRx-HA

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

HA

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2

Plasmid

#231372

Purpose

Express human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Human, Synthetic)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_λHA-GFPopt

Plasmid

#210575

Purpose

Expresses lambdaHA-optimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-GFP optimal

Tags

lambda HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_λHA-GFPnonopt

Plasmid

#210576

Purpose

Expresses lambdaHA-nonoptimal GFP

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-GFP nonoptimal

Tags

lambda HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I TAA

Plasmid

#208187

Purpose

nonsense mistranslation, TAA stop

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Expression

Bacterial

Mutation

Insertion 6TAA*(stop) 7AGT(Ser)

Promoter

Ptet+dnaK P1

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCBP60g-NLS-3xmCitrine

Plasmid

#192521

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

CALMODULIN BINDING PROTEIN 60g

Tags

3xmCitrine and NLS

Expression

Plant

Promoter

CBP60g

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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p5E_ngn1_LSE_C

Plasmid

#199395

Purpose

pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

LSE_C

Use

Gateway Cloning

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha2_SulR

Plasmid

#186425

Purpose

transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Insert

SulR

Use

Synthetic Biology; Binary vector for escherichia …

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDGB1alpha11_SulR

Plasmid

#186424

Purpose

transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assembly

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Insert

SulR

Use

Synthetic Biology; Binary vector for escherichia …

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pVC

Plasmid

#175063

Purpose

expresses mVenus and mCherry pair in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

mVenus + mCherry

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-LuxA

Plasmid

#188305

Purpose

Expression of LuxA via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

luxA

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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