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MAC_N_RAB11A

Plasmid

#111691

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

RAB11A (RAB11A Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETa-Amrose v1

Plasmid

#79537

Purpose

pRSETa (Invitrogen) plasmid expressing Amrose variant 1

Depositor

Randolph Caldwell

Article

Schoetz et al PLoS One. 2014 Sep 5;9(9):e107069. doi:

Insert

Amrose

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc Rheb Omp25

Plasmid

#112764

Purpose

express myc-Rheb-Omp25

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

myc-Rheb-Omp25 (RHEB Human)

Tags

Omp25 and myc

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-H-Ras_V12

Plasmid

#39510

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 (HRAS Human)

Use

Eukaryotic

Mutation

G12V

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
LZRSpBMN-Z‐GFP‐Eps8

Plasmid

#74935

Purpose

Expresses mouse Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
pDSM-de-Pfba1-PYC-Trpl15a

Plasmid

#127738

Purpose

Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfba1

Available Since

Jan. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

Plasmid

#82469

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mRNA capping enzyme (Rngtt Mouse)

Tags

Flag and Myc

Expression

Mammalian

Mutation

changed Lysine 294 to Alanine

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-myc NES-mCE NLS-Flag

Plasmid

#82468

Purpose

Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-NES-mCE-Flag (Rngtt HIV, Mouse)

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1C389S-HLx8

Plasmid

#108138

Purpose

Recruitable inactive SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1LC389S(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKD265

Plasmid

#125095

Purpose

Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torR(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSR322

Plasmid

#125116

Purpose

Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.15

Plasmid

#125082

Purpose

Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.29

Plasmid

#125088

Purpose

Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)139
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) NEMO shRNA1

Plasmid

#22505

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA1 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
mTFP1-Vimentin-7

Plasmid

#55515

Purpose

Localization: Intermediate Filaments, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Vimentin (VIM Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
GAG-PKB-pcDNA3

Plasmid

#24651

Depositor

Jim Woodgett

Article

Jones et al J Exp Med. 2000 May 15. 191(10):1721-34.

Insert

PKB (AKT1 Human)

Tags

GAG

Expression

Mammalian

Available Since

June 7, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pDSM-ab-Psptef1-NAT-Ttip1

Plasmid

#127747

Purpose

Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

nourseothricin N-acetyltransferase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psptef1

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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USH1C_PDZ_1

Plasmid

#103936

Purpose

Protein expression and purification of P73 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

USH1C (USH1C Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMflPT-o4

Plasmid

#101315

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and pac resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
pac resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMflST-o4

Plasmid

#101316

Purpose

Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and aadA1 resistance genes, origin of transfer of RP4 plasmid.

Depositor

Sébastien Rodrigue

Article

Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

Inserts

oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
aadA1 resistance gene

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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