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pCMV4a-SIRT2-Flag

Plasmid

#102623

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

Sirtuin 2 (SIRT2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer20 EGFP-TEADi

Plasmid

#140145

Purpose

Tetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

EGFP TEADi

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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p3HD1RLuc2

Plasmid

#236276

Purpose

Mammalian expression of human dopamine D1 receptor; N-terminal HA tag, C-terminal Rluc8

Depositor

Vsevolod Gurevich

Article

Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

Insert

dopamine D1 receptor (DRD1 Human)

Tags

3xHA and Rluc8

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6-CFAPoptim-KAN

Plasmid

#221128

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

FAPoptim

Expression

Yeast

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-TetON-Hygro-Arhgef11-Cry2-mCh

Plasmid

#240798

Purpose

Dox-inducible expression of Arhgef11-Cry2-mCherry

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

Arhgef11-Cry2-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Promoter

TRE

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-p54

Plasmid

#35379

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

p54 (NONO Human)

Tags

FLAG

Expression

Mammalian

Mutation

A464V

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag_fast

Plasmid

#167275

Purpose

Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO

Plasmid

#86696

Purpose

Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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HA-Halo-HSF2

Plasmid

#200202

Purpose

Expression of full length HSF2

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HA-Halo-HSF2 (Hsf2 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-mRFP1

Plasmid

#112914

Purpose

Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

mRFP1

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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His-MBP_G4G6 in pRSF

Plasmid

#188454

Purpose

Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

G4G6 (GSN Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

Plasmid

#232183

Purpose

Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6, hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynLAMA-F98 IRES EGFP

Plasmid

#130710

Purpose

Plasmid encoding outer inner membrane localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-LAMA-F98 IRES EGFP

Tags

Lyn11 and SNAP-Tag

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagA

Plasmid

#112740

Purpose

express FRB-myc-RagA

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagA (RRAGA Human)

Use

Lentiviral

Tags

FRB, myc

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagC

Plasmid

#112741

Purpose

express FRB-myc-RagC

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagC (RRAGC Human)

Use

Lentiviral

Tags

FRB, myc

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pUTKan-3xHA-GFP-TOM5

Plasmid

#130674

Purpose

Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

Depositor

Andreas Weber

Article

Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

Insert

Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)

Tags

3xHA and sGFP

Expression

Plant

Promoter

Ubiquitin10

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (STOP)99xGly

Plasmid

#211349

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codon

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(STOP)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

Start (ATG) codon replaced with stop (TAG) codon

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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