We narrowed to 5,950 results for: chia
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Plasmid#30028DepositorInsertpLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialAvailable SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3612 (=HL 716 + pHL 841)
Plasmid#30082DepositorInsertpLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialAvailable SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3619 (=HL 716 + pHL 1228)
Plasmid#30083DepositorInsertpLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialAvailable SinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL7 SMR1791
Plasmid#28030DepositorAvailable SinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV Ub Lex VP16 (F442A) HA (P#1711)
Plasmid#14596DepositorInsertLexA
TagsHA, Ubiquitin, and VP16 (F442A)ExpressionMammalianMutationThe VP16 sequence contains an F442A mutation. The…Available SinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Nano
Plasmid#60409PurposeE. coli expression of SspB NanoDepositorInsertSspB nano
TagsHis-MBP-TEVExpressionBacterialMutationY11K and A15EAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
ExpressionMammalianPromoterU6Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
TagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialPromoterpDABAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
TagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialPromoterT7Available SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgRNA(F+E)-sgGal4
Plasmid#100549PurposesgGal4-expressing vector modified from Addgene plasmid #51024DepositorInsertsgGal4
UseCRISPRTagssgRNAExpressionMammalianPromoterU6Available SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Dead Streptavidin-SpyTag
Plasmid#59548PurposepET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramersDepositorInsertDead Streptavidin-SpyTag
TagsSpyTagExpressionBacterialMutationThe construct lacks final K of SpyTagPromoterT7Available SinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDOC-GG
Plasmid#149377PurposeChassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene DoctoringDepositorInsertsFragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRExpressionBacterialPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
ExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-SCR-sgRNA-A
Plasmid#177811PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
ExpressionBacterialAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
aav-CAG-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113761PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterCAGAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable SinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only