We narrowed to 23,650 results for: crispr

1. pHR-SFFV-dCas9-BFP-KRAB

   Plasmid

   #46911

   Purpose

   Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-BFP-KRAB fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2xNLS, BFP, HA, and KRAB domain

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC101-Donor

   Plasmid

   #140630

   Purpose

   Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   LE, RE, KanR, temperature sensitive pSC101 origin of replication
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ141-ZmUbi-RZ-As

   Plasmid

   #86196

   Purpose

   AsCpf1 gRNA entry plasmid using Zea mays Ubi promoter and ribozyme processing

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   AsCpf1 gRNA cloning site for ribozyme cleavage
   

   Use

   CRISPR

   Tags

   Hammerhead ribozyme and HDV ribozyme

   Expression

   Plant

   Promoter

   Maize ubiquitin 1

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

   Plasmid

   #64216

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEL13

   Plasmid

   #107919

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMEL12

   Plasmid

   #107918

   Purpose

   hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-SFFV-KRAB-dCas9-P2A-mCherry

    Plasmid

    #60954

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Insert

    KRAB-dCas9-P2A-mCherry fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, KRAB domain, and mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ-CBE-SpRYn-Act3.0

    Plasmid

    #178958

    Purpose

    It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. non-targeting control gRNA (BRDN0001487130)

    Plasmid

    #80262

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. non-targeting control gRNA (BRDN0001147380)

    Plasmid

    #80225

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ1651-sgTelomere(F+E)

    Plasmid

    #51024

    Purpose

    Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    Optimized sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-dMSK1

    Plasmid

    #165602

    Purpose

    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 S. Pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-ddMSK1

    Plasmid

    #165603

    Purpose

    Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

    Depositor

    Isaac Hilton

    Article

    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

    Insert

    S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 S. Pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG Tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. non-targeting control gRNA (BRDN0001148862)

    Plasmid

    #80263

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pROS10

    Plasmid

    #107924

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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