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pNOC_hfnCas12a-Nlux

Plasmid

#176239

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Promoter

Ribosomal subunit

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript II KS(+) ACMV DNA-A 1.4mer

Plasmid

#159134

Purpose

Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-A

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-A 1.4mer

Use

Infectious clone for bombarding plants

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP-Dronpa

Plasmid

#87709

Purpose

FKBP-tagged Dronpa.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FKBP-Dronpa

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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mTFP1-VSVG-16

Plasmid

#55517

Purpose

Localization: Golgi/ER Transport, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

VSVG

Tags

mTFP1

Expression

Mammalian

Mutation

deltaK VSVG ts045

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-N1_MeCP2(deltaNC)

Plasmid

#110190

Purpose

Expression of mouse MeCP2 (deltaNC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pUHG10.3-TetO-Rs1

Plasmid

#16313

Depositor

Bruce Conklin

Article

Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

Insert

h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Added FLAG tag; Changed Aspartic Acid 100 to Alan…

Available Since

Jan. 3, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mScarlet (AAV8)

Viral Prep

#131001-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-hSyn-mScarlet (#131001). In addition to the viral particles, you will also receive purified pAAV-hSyn-mScarlet plasmid DNA. Synapsin-driven mScarlet control vector. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Promoter

Syn

Tags

mScarlet

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only
pPH36

Plasmid

#35101

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fca1MX4

Use

Yeast replicating

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-CK(1.3)GW

Plasmid

#27227

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

AAV

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon5

Plasmid

#217456

Purpose

For TP53 knockout targeting exon 5 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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PX459-TP53-exon4

Plasmid

#217455

Purpose

For TP53 knockout targeting exon 4 of TP53

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for TP53 (TP53 Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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Clomeleon

Plasmid

#203723

Purpose

A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

Depositor

George Augustine

Article

Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

Insert

cyan fluorescent protein linked yellow florescent protein

Expression

Bacterial and Mammalian

Mutation

Cyan Fluorescent Protein, Topaz Fluorescent Prote…

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Mkk7-JNK2 fusion-pcw107

Plasmid

#64618

Purpose

Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

Map2k7 linker MAPK9

Use

Lentiviral

Tags

FLAG

Mutation

mouse-human fusion

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pCfB8787

Plasmid

#126921

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

HMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAG-3xNLS-vsfGFP-0

Plasmid

#191097

Purpose

To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-vsfGFP-0

Use

AAV

Tags

Green fluorescent protein bound to enhancer nanob…

Expression

Mammalian

Mutation

Optimized to Human codon usage

Promoter

CMV and CAG

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJK1

Plasmid

#111722

Purpose

to express human BLM protein in yeast cells

Depositor

Ian Hickson

Article

Karow et al J Biol Chem. 1997 Dec 5;272(49):30611-4.

Insert

BLM (BLM Human)

Tags

hexahistidine

Expression

Yeast

Mutation

a C-terminal hexahistidine tag was added, a yeast…

Promoter

GAL1

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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hHS1-M7A,H102A

Plasmid

#159171

Purpose

Human codon-optimized cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

hHS1-M7A,H102A

Expression

Mammalian

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD∆cat

Plasmid

#81054

Purpose

bacterial expression of catalytic dead catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057 with histid…

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pAAV-hSYN1-NES-OCaMP-WPRE

Plasmid

#224934

Purpose

pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSynI

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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