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Plasmid#209914PurposeTo monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent proteinDepositorInsertEREG-mNeonGreen (EREG Human)
UseLentiviralTagsmNeonGreenExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-TGFa-ScNeo
Plasmid#209904PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-NRG1-ScNeo
Plasmid#209906PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNRG1-ScNeo (NRG1 Human)
UseTagsmNeonGreen and mScarletExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-TGFa-ScNeo
Plasmid#209908PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
(attB) tdMCP-tagged BRaf
Plasmid#226165PurposeUsed to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.DepositorInsert3xFlag-tdMCP-BRaf (BRAF Human)
UseBxb1 attb, no promoterTags3xFlag-tdMCP and IRES-mCherry-P2A-PuroRExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DXO
Plasmid#177993PurposeFor purification of N-terminally His-tagged DXO from bacteriaDepositorInsertDXO (DXO Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-blue
Plasmid#223532PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsNS3 Hepatitis C protease and mTurquoiseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-NS3-Green
Plasmid#223440PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP3-NS3-Blue
Plasmid#223533PurposeMammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.DepositorInsertCHMP3 (CHMP3 Human)
UseTagsNS3 Hepatitis C protease and mTurquoiseExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP3-mut-NS3-Green
Plasmid#223530PurposeMammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP3 (CHMP3 Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP2A-mut-NS3-Green
Plasmid#223529PurposeMammalian expression of CHMP2A fused to the hepatitis C virus protease NS3 through a short linker containing a mutated NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP2A (CHMP2A Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHMP3-NS3-Green
Plasmid#223527PurposeMammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP3 (CHMP3 Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-Doc2β C2AB (G361C)
Plasmid#213631PurposeBacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)DepositorInsertDoc2β C2AB (G361C) (Doc2b Rat)
UseTagsSUMOExpressionBacterialMutationG361C, all original cysteines mutated to alaninesPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 WT
Plasmid#212946PurposeVector used for expression of AtSLAC1 WT in mammalian cellDepositorInsertSlow anion channel-associated 1 (OZS1 Mustard Weed)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00036 (aka pMC0223)
Plasmid#212659PurposeGuide RNA targeting SERPINB3DepositorInsertSERBPINB3 guide RNA (SERPINB3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00033 (aka pMC0224)
Plasmid#210750PurposeGuide RNA targeting SERPINB3DepositorInsertSERBPINB3 guide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W
Plasmid#208552PurposeLentiviral vector expressing gRNA targeting human RUNX3DepositorInsertRUNX3(13) (RUNX3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W
Plasmid#208551PurposeLentiviral vector expressing gRNA targeting human RUNX3DepositorInsertRUNX3(12) (RUNX3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(AAVS1)-PGKpuro2ABFP-W
Plasmid#200459PurposeLentiviral vector expressing gRNA targeting human AAVS1DepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W
Plasmid#210612PurposeLentiviral vector expressing gRNA targeting a control region at the CXCR4 locusDepositorInsertControl(1)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR
Plasmid#211820PurposeLentiviral vector expressing a dead GFP adenine base editing reporterDepositorInsertdead GFP reporter
UseLentiviralTagsExpressionMammalianMutationPromoterCMV promoter and SV40 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ08-pAAVsc.U6-tracrRNA
Plasmid#211816PurposeAAV vector expressing tracrRNA under U6 promoterDepositorInsertU6-tracrRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W
Plasmid#200491PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(16) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W
Plasmid#200490PurposeLentiviral vector expressing gRNA targeting human RIC8ADepositorInsertRIC8A(15) (RIC8A Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W
Plasmid#210613PurposeLentiviral vector expressing gRNA targeting a control region at the CXCR4 locusDepositorInsertControl(2)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003(V114T, V116T)-Talin rod-mCherry
Plasmid#210027PurposeExpression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cellsDepositorInsertSpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
UseTagsExpressionMammalianMutationSpyTag003 V114T and V116T mutations to modify Spy…PromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mNeonGreen -IRES- Talin head-SpyTag003
Plasmid#210024PurposeExpression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cellsDepositorInsertLifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
UseTagsExpressionMammalianMutationEncephalomyocarditis virus internal ribosome entr…PromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003(K31TAG)
Plasmid#210023PurposeExpression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cellsDepositorInsertEGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
UseTagsExpressionMammalianMutationSpyCatcher003 with K31TAG (Amber stop codon) muta…PromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A_R
Plasmid#200183PurposeWT PA14 I-F Leader and G1A-T2A RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A)
UseTagsExpressionBacterialMutationG1A-T2A in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT_IFL_G1A-T2A-A28C_R
Plasmid#200185PurposeWT PA14 I-F Leader and G1A-T2A-A28C RepeatDepositorInsertWildtype I-F Leader and a mutant I-F Locus (G1A-T2A-A28C)
UseTagsExpressionBacterialMutationG1A-T2A-A28C in the I-F repeatPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only