We narrowed to 60,982 results for: Ina

1. His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

   Plasmid

   #187727

   Purpose

   Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 226-382 removed (NEDD4L Human)
   

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 226-382

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

   Plasmid

   #187728

   Purpose

   Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 418-495 removed (NEDD4L Human)
   

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 418-495

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

   Plasmid

   #187711

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 with mutations (NEDD4L Human)
   

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491; mutations C702A, C776…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1-NEDD4L_Δ1,2-linker

   Plasmid

   #187714

   Purpose

   Express GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 226-382 removed (NEDD4L Human)
   

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 226-382

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1-NEDD4L_Δ2,3-linker

   Plasmid

   #187715

   Purpose

   Express GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 418-495 removed (NEDD4L Human)
   

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 418-495

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. JO-pH720-SARS-CoV-2-S-tail-WT

   Plasmid

   #218453

   Purpose

   Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
   

   Tags

   eXact

   Expression

   Bacterial

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FENEF-Sensor

   Plasmid

   #208930

   Purpose

   in vitro p38-Sensor for luminescent detection of p38-activity

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   FENEF sequence from human ATF2 with phospho site (ATF2 Human)
   

   Tags

   HISx6, MBP, and Smbit

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV5D HA-Δ37, 115-133, 204-217 PPM1H

   Plasmid

   #207344

   Purpose

   Mammalian expression of HA-Δ37, 115-133, 204-217 PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Δ37, Δ115-133, Δ204-217

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAGGS-safb∆rrm-NLS-3XFLAG-V5

   Plasmid

   #196093

   Purpose

   Expresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Removed amino acids 428-506 (RRM) of mouse SAFB p…

   Promoter

   CAGGS

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB1N-mApple-LZ-LOV2

    Plasmid

    #197418

    Purpose

    Mammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Curr Biol. 2022 Mar 14;32(5):1197-1205.e

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Tags

    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. kanMX6-ins1

    Plasmid

    #195038

    Purpose

    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

    Plasmid

    #180314

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 )
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    SEPT9_i1-i5

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_SEPT9_i1 S22A S23A-msfGFP

    Plasmid

    #180338

    Purpose

    mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S22A S23A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_SEPT9_i1 R10A R15A-msfGFP

    Plasmid

    #180336

    Purpose

    mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 R10A R15A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_SEPT9_i1 S12A S13A-msfGFP

    Plasmid

    #180337

    Purpose

    mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 S12A S13A

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUMO GLTP

    Plasmid

    #170732

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    GLTP (GLTP Human)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti_CRISPRi_sgAR10

    Plasmid

    #167003

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 10 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.1 mitoAURKA Lys162Met

    Plasmid

    #157752

    Purpose

    Expression of kinase-dead AURKA K62M in mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRC/CMV-TYK2(delta)KL-VSV

    Plasmid

    #139352

    Purpose

    Expression of TYK2 protein missing the kinase-like (pseudokinase) domain

    Depositor

    Sandra Pellegrini

    Article

    Velazquez et al J Biol Chem. 1995 Feb 17;270(7):3327-34.

    Insert

    TYK2 cDNA deleted of aa594-877 with a Tyr inserted after aa593 (TYK2 Human)
    

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-deletion of aa 594-877

    Promoter

    CMV

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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