We narrowed to 37,839 results for: Tec

1. pRSET-mSA-EGFP

   Plasmid

   #39862

   Depositor

   Sheldon Park

   Article

   Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

   Insert

   monomeric streptavidin fused to EGFP
   

   Use

   Tags

   6xHis, EGFP, and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSCX

   Plasmid

   #63918

   Purpose

   Expression of the sucrose utilization phenotype

   Depositor

   Claudia Vickers

   Article

   Bruschi et al Biotechnol Adv. 2012 Sep-Oct;30(5):1001-

   Insert

   cscAKB
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV anti-FITC-LaG16-sNRR-T2A-BFP

   Plasmid

   #194930

   Purpose

   LV construct encoding sNRR-based SynNotch receptor

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   antiFITC-LaG16-sNRR-SynNotch-Gal4-VP64-T2A-BFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PX851

   Plasmid

   #62883

   Purpose

   N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX853

   Plasmid

   #62885

   Purpose

   N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-573)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLM802

   Plasmid

   #27203

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMLM800

   Plasmid

   #27202

   Depositor

   Keith Joung

   Article

   Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

   Insert

   FokI endonuclease
   

   Use

   Zebrafish expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXL-CAG-ICeuI-sHRPaNRX3beta

   Plasmid

   #79910

   Purpose

   piggyBac transposon vector, sHRPaNRX3beta

   Depositor

   Joshua Sanes

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPaNRX3beta
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. FB430

   Plasmid

   #203621

   Purpose

   TU for the expression of phleomycin resistance.

   Depositor

   Jose Marcos

   Article

   Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

   Insert

   PpcbC:ble:TamdS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FB431

    Plasmid

    #203622

    Purpose

    TU for the expression of terbinafine resistance.

    Depositor

    Jose Marcos

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    PgpdA:ergA:TamdS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV anti-FITC-LaG16-Y186A-T2A-BFP

    Plasmid

    #194932

    Purpose

    LV construct encoding Y186A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LV anti-FITC-LaG16-Y192A-T2A-BFP

    Plasmid

    #194933

    Purpose

    LV construct encoding Y192A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 Ras-LOCKR-S

    Plasmid

    #216496

    Purpose

    FRET-based reporter for Ras activity, untargeted

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBacDual with PA+Histag

    Plasmid

    #84870

    Purpose

    Baculovirus Expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Baculovirus

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET22b - PA

    Plasmid

    #84863

    Purpose

    bacterial expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Tags

    pelB signal sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-6xHis-AviTag-ALFAtag-eGFP

    Plasmid

    #195854

    Purpose

    Bacterial expression vector encoding 6xHis-AviTag-ALFAtag-eGFP. Co-expression in cells with biotin ligase (BirA) results in efficient biotinylation of the AviTag sequence.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    6xHis-AviTag-ALFAtag-eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX852

    Plasmid

    #62884

    Purpose

    C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS SV40, and NLS nucleoplasmin

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge