We narrowed to 33,824 results for: URE

1. ReaLiSM A1-26link-GPIba+2cys

   Plasmid

   #59580

   Purpose

   express VWF A1-GPIba ReaLiSM construct in mammalian cells

   Depositor

   Timothy Springer

   Article

   Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

   Insert

   VWF A1-linker-GPIba (VWF Human)
   

   Use

   Tags

   6His

   Expression

   Mammalian

   Mutation

   Promoter

   chick beta-actin

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pLVX-Flag-MIB1-IRES-Puro

   Plasmid

   #140240

   Purpose

   Lentiviral vector expressing flag-tagged MIB1

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Insert

   Flag-MIB1 (MIB1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. shRNA_circHUWE1_1

   Plasmid

   #215236

   Purpose

   Supression of shcircHUWE1(19,20)_1 expression

   Depositor

   Sefi Rosenbluh

   Article

   Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

   Insert

   circHUWE1 shRNA 1 (HUWE1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. shRNA_circHUWE1_2

   Plasmid

   #215235

   Purpose

   Supression of shcircHUWE1(19,20)_2 expression

   Depositor

   Sefi Rosenbluh

   Article

   Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

   Insert

   circHUWE1 shRNA 2 (HUWE1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST_FLAG-HUWE1_UBA/UIM/ UBM-mut

   Plasmid

   #187154

   Purpose

   mammalian cell expression of FLAG-HUWE1 L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   L2976A/P2977A/M1328A/F1330A/A1376G/S1382A

   Promoter

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIneoRL-BCL2 3'UTR-mir200c-mut

   Plasmid

   #84598

   Purpose

   Renilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   BCL2 3'UTR (UTS2R Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mutation

   Mutation on miR-200c binding site on BCL2 3'…

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCIneoRL-TOB1 3'UTR-mir200c-mut

   Plasmid

   #84590

   Purpose

   Renilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   TOB1 3'UTR (UTS2R Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mutation

   Mutation on miR-200c binding site on TOB1 3'…

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

8. P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

   Plasmid

   #239972

   Purpose

   Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

   Plasmid

   #239974

   Purpose

   PiggyBac plasmid for inducible PEmax expression and Puromycin selection

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Rlucrarr3-Flash5

    Plasmid

    #74133

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Rlucrarr3-Flash6

    Plasmid

    #74134

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Rlucrarr3-Flash1

    Plasmid

    #74129

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. Rlucrarr3-Flash4

    Plasmid

    #74132

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 225 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

15. Rlucrarr3-Flash3

    Plasmid

    #74131

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

16. Rlucrarr3-Flash2

    Plasmid

    #74130

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 139 of Arrestin3.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pRMC2-sec:msfGFP

    Plasmid

    #194914

    Purpose

    Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human a4 nicotinic subunit crystallization construct

    Plasmid

    #85841

    Purpose

    pEZT-BM vector with alpha4 gene after CMV promoter, published crystallization construct

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

    Insert

    Human a4 nAChR subunit crystallization construct (CHRNA4 Human)
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Human b2 nicotinic subunit crystallization construct

    Plasmid

    #85842

    Purpose

    pEZT-BM vector with beta2 gene after CMV promoter, published crystallization construct

    Depositor

    Ryan Hibbs

    Article

    Morales-Perez et al Nature. 2016 Oct 3;538(7625):411-415. do

    Insert

    Human b2 nAChR subunit crystallization construct (CHRNB2 Human)
    

    Use

    Baculovirus (bacmam) production and mammalian exp…

    Tags

    C-terminal StrepII tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRMC2-tat:msfGFP

    Plasmid

    #194915

    Purpose

    Tetracycline inducible expression of msfGFP fused to Tat signal peptide in Staphylococci

    Depositor

    Rikke Meyer

    Article

    Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

    Insert

    Shine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
    

    Use

    Tetracycline-inducible expression vector for stap…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge