We narrowed to 6,744 results for: WUS

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pCMV-Vamp2-pHluorin

Plasmid

#190151

Purpose

Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

superecliptic pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-ASF1B

Plasmid

#31205

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ASF1B (ASF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)

Plasmid

#105200

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-P2A-antiPD1(LC)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-BoxB

Plasmid

#92004

Purpose

Expresses firefly luciferase with BoxB sequences in 3' UTR

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Firefly Luciferase

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_HNF4A_P2A_Hygro_Barcode

Plasmid

#120450

Purpose

Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HNF4A (HNF4A Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-mKate2.5f

Plasmid

#99129

Purpose

Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mKate2.5f

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX_hDDX5_FL_wt

Plasmid

#88871

Purpose

inducible expression of GST-DDX5 in bacteria

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9C

Plasmid

#80931

Purpose

Expresses Cas9C in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9C

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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His6-pAG-RTase

Plasmid

#214932

Purpose

Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

Depositor

Chuan He

Article

Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

Insert

fusion protein of protein A/G and MMLV reverse transcriptase (25-497)

Tags

His6

Expression

Bacterial

Mutation

MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

Promoter

T7 promoter

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Fis1

Plasmid

#182580

Purpose

Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Fis1

Tags

Fis1

Expression

Mammalian

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3

Plasmid

#108103

Purpose

Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Ubc13-WT

Plasmid

#43938

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Tags

2x FLAG

Expression

Mammalian

Available Since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-NCAPH

Plasmid

#31214

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NCAPH (NCAPH Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

Plasmid

#101299

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-698 and 698-906 of alpha-C…

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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LV-AR

Plasmid

#213819

Purpose

Lenti-viral expression of human androgen receptor wild-type (WT).

Depositor

Steven Balk

Article

Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

Insert

AR (human androgen receptor) (AR Human)

Use

Lentiviral

Tags

Flag (N terminal on backbone)

Expression

Mammalian

Mutation

No, it is AR wild-type.

Promoter

EF-1a

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

Plasmid

#132400

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a_GATA6_P2A_Hygro_Barcode

Plasmid

#120445

Purpose

Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA6 (GATA6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-tdTomato

Plasmid

#99116

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein tdTomato

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

DIO-tdTomato

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-EGFP

Plasmid

#91975

Purpose

EGFP reporter cell line control (no miR-19 sites)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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