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Plasmid#158016PurposeExpression vector for the de novo designed protein Expression_vector_RO2_5DepositorInsertde novo designed protein RO2_5
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_6
Plasmid#158017PurposeExpression vector for the de novo designed protein Expression_vector_RO2_6DepositorInsertde novo designed protein RO2_6
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_9
Plasmid#158018PurposeExpression vector for the de novo designed protein Expression_vector_RO2_9DepositorInsertde novo designed protein RO2_9
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_10
Plasmid#158019PurposeExpression vector for the de novo designed protein Expression_vector_RO2_10DepositorInsertde novo designed protein RO2_10
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_15
Plasmid#158020PurposeExpression vector for the de novo designed protein Expression_vector_RO2_15DepositorInsertde novo designed protein RO2_15
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_20
Plasmid#158021PurposeExpression vector for the de novo designed protein Expression_vector_RO2_20DepositorInsertde novo designed protein RO2_20
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_RO2_25
Plasmid#158022PurposeExpression vector for the de novo designed protein Expression_vector_RO2_25DepositorInsertde novo designed protein RO2_25
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_NT_1
Plasmid#158023PurposeExpression vector for the de novo designed protein Expression_vector_NT_1DepositorInsertde novo designed protein NT_1
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_NT_8
Plasmid#158024PurposeExpression vector for the de novo designed protein Expression_vector_NT_8DepositorInsertde novo designed protein NT_8
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_NT_9
Plasmid#158025PurposeExpression vector for the de novo designed protein Expression_vector_NT_9DepositorInsertde novo designed protein NT_9
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Expression_vector_NT_10
Plasmid#158026PurposeExpression vector for the de novo designed protein Expression_vector_NT_10DepositorInsertde novo designed protein NT_10
UseTags6x His tagExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4G
Plasmid#104502PurposeUsed to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter geneDepositorInsertScPGM2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4K
Plasmid#104506PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSpGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4O
Plasmid#104510PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSpdGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4P
Plasmid#104511PurposeUsed to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSptGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEC3105 (pSpy1C-PxylS2_ffluc)
Plasmid#218512Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPxylS2Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS178
Plasmid#215678PurposeEmpty repair plasmid for ChrII split hygromycinR landing padDepositorInsert5'HA + MCS + loxP + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS114
Plasmid#215677PurposeEmpty repair plasmid for ChrI split hygromycinR landing padDepositorInsert5'HA + MCS + loxN + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS59
Plasmid#215680PurposeCas9 + guide plasmid targeting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only