We narrowed to 6,996 results for: nsf

1. pCSm5En2CER4(#457)

   Plasmid

   #184090

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-chkEn2(C>S)-ERT2
   

   Use

   Synthetic Biology

   Tags

   5xMyc

   Expression

   Mammalian

   Mutation

   chkEn2: C175S; ERT2: G400V, M543A, L544A, deleted…

   Promoter

   sCMV+SP6

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSS-GFP

   Plasmid

   #163661

   Purpose

   Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from TNF and TMD and luminal domain from ST6GAL1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from TNF…

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SNN-GFP

   Plasmid

   #163664

   Purpose

   Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TNF in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from ST,…

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. NNS-GFP

   Plasmid

   #163666

   Purpose

   Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TNF, and luminal domain from ST6GAL1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This chimera contains the cytosolic tail from TNF…

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSUMO GLTP

   Plasmid

   #170732

   Purpose

   The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

   Depositor

   Rhoderick Brown

   Article

   Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

   Insert

   GLTP (GLTP Human)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNIA-CEN-FLAG-LANS1-Set2

   Plasmid

   #122002

   Purpose

   FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

   Depositor

   Brian Kuhlman

   Article

   Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

   Insert

   histone methyltransferase SET2 (SET2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Set2 point mutations K538G, K539S, R549G, K550S, …

   Promoter

   ADH1

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK215

   Plasmid

   #72427

   Purpose

   Acetobacter aceti 1023 gDNA

   Depositor

   T. J. Kappock

   Inserts

   succinyl-diaminopimelate desuccinylase
   2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
   acetylglutamate kinase
   ribosome biogenesis GTP-binding protein YsxC
   insertase
   ribonuclease P
   50S ribosomal protein L34
   hypothetical protein
   glyoxylase I
   hypothetical protein
   UDP-N-acetylglucosamine acyltransferase
   3-hydroxyacyl-ACP dehydratase
   UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
   

   Use

   Phage lambda cloning vector with genomic dna inse…

   Tags

   Expression

   Mutation

   missing C-terminal domain residues 277-391(ter) a…

   Promoter

   n/a

   Available since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF_his6_sumo_ZIKV_NS5_MTase

   Plasmid

   #213529

   Purpose

   Expresses the MTase domain of NS5 of ZIKV

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   ZIKA virus NS5 methyltransferase domain
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   MTase domain aa 1-265

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV10-3xFLAG-prs-hMIGA2(170-593)-25GS-6xhis

   Plasmid

   #192835

   Purpose

   Express codon-optimized human MIGA2 fragment 170-593 with a GS-linker and 6xhis tag at C-terminus (lipid transfer construct) in Expi293 cells

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Use

   Tags

   3xFLAG-PreScission cleavage site and 6xhis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSmEn2n4ERT(#234)

    Plasmid

    #184082

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(n4)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

    Promoter

    sCMV+SP6

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSmEn2SRERT(#231)

    Plasmid

    #184080

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(SR)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

    Promoter

    sCMV+SP6

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCSmEn25EERT(#232)

    Plasmid

    #184081

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    6his-myc-chkEn2(5E)-ERT2
    

    Use

    Synthetic Biology

    Tags

    6his-Myc

    Expression

    Mammalian

    Mutation

    chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

    Promoter

    sCMV+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-DUET1-DHDDS/Sumo-NgBR

    Plasmid

    #203162

    Purpose

    Expression of human cis-prenyltransferase complex for purification

    Depositor

    William Sessa

    Article

    Edani et al Proc Natl Acad Sci U S A. 2020 Aug 25;11

    Inserts

    DHDDS (DHDDS Human)
    NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    truncation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDCAF3-IS5

    Plasmid

    #65220

    Purpose

    Expresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.

    Depositor

    Jeffrey Barrick

    Article

    Quandt et al ACS Synth Biol. 2013 Jun 21;2(6):301-7.

    Inserts

    ndmA
    ndmB
    ndmC
    ndmD
    gst9
    chlR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed start codon from gtg to atg and Changed s…

    Promoter

    BBa_J23100

    Available since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid

    #169191

    Purpose

    To express nsp10-nsp16 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497

    Insert

    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag

    Plasmid

    #210021

    Purpose

    Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface.

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    Transferrin receptor transmembrane domain-SpyCatcher003(K31TAG)-superfolderGFP-MycTag-CTag (TFRC Human, Synthetic)
    

    Use

    Tags

    MycTag, CTag

    Expression

    Mammalian

    Mutation

    Transferrin receptor transmembrane domain with Y2…

    Promoter

    CMV promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-72B1

    Plasmid

    #59374

    Purpose

    Inducible expression of 71B2 (Q9M156) under T7 promoter

    Depositor

    Christopher Anderson

    Insert

    UGT72B1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. YCpADE1

    Plasmid

    #22494

    Depositor

    Beth Montelone

    Insert

    ADE1 (ADE1 Budding Yeast)
    

    Use

    Yeast shuttle

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBAD-MBP-His-SFP

    Plasmid

    #141141

    Purpose

    Expression of 4'-phosphopantetheinyl transferase gene

    Depositor

    New England Biolabs

    Insert

    4'-phosphopantetheinyl transferase
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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