We narrowed to 18,766 results for: nts

1. pRPL28 SpHIS5 (pNTI663)

   Plasmid

   #115433

   Purpose

   Expresses wild-type RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Expression

   Yeast

   Mutation

   MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRPL28(Q38P) SpHIS5 (pNTI664)

   Plasmid

   #115434

   Purpose

   Expresses Q38P RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Expression

   Yeast

   Mutation

   Q38P; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRPL28(H39L) SpHIS5 (pNTI665)

   Plasmid

   #115435

   Purpose

   Expresses H39L RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Expression

   Yeast

   Mutation

   H39L; MfeI site in intron

   Promoter

   ashbya gospii TEF1 and endogenous

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBW1944_ hU6-PV4-loxP-BbsI-ENTRY-gRNA

   Plasmid

   #87572

   Purpose

   PV4 gRNA entry vector for CRISPR decoder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW1943_ hU6-PV3-FRT-loxP-BbsI-ENTRY-gRNA

   Plasmid

   #89056

   Purpose

   PV3 gRNA entry vector for CRISPR decoder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBW759_pCAG-PV2-lox2272-GFP-BGHpA

   Plasmid

   #87575

   Purpose

   PV2 for 2-input, 4-output decoder circuit and 3-input, 2-output Full Adder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW758_pCAG-PV1-VloxP-FRT-lox2272-NeoR-BGHpA

   Plasmid

   #87580

   Purpose

   PV1 for 3-input, 2-output Full Adder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   Neomycin Resistance Gene
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAD06 K. lactis telomerase RNA hairpin

   Plasmid

   #61895

   Purpose

   Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

   Depositor

   Thomas Cech

   Article

   Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

   Insert

   Telomerase RNA hairpin (nt 229-273)
   

   Promoter

   T7

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-MAVS-M1A500(KaganF60)

   Plasmid

   #52003

   Purpose

   Expresses the human MAVS CDS containing a point mutation M1A and a C-terminal deletion removing the transmembrane domain

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M1A-500
   

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala and deleted the C-terminal t…

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)

    Plasmid

    #52018

    Purpose

    Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M1A,M142A
    

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala and Met 142 to Ala

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMSCV-MAVS-M1A,M142A(KaganE20)

    Plasmid

    #52011

    Purpose

    Expresses the human MAVS CDS containing the point mutations M1A and M142A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M1A,M142A
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala and Met 142 to Ala

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid

    #49062

    Purpose

    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    N-terminal deletion of amino acids 13-222 in hNKC…

    Promoter

    CMV

    Available Since

    Nov. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4G-Cloning Plasmid Kit

    Plasmid Kit

    #1000000252

    Purpose

    The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.

    Depositor

    Stephan Gruber

    Available Since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-LRF

    Plasmid

    #66936

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pGL3-LRR

    Plasmid

    #66937

    Purpose

    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).

    Depositor

    Alvaro Monteiro

    Article

    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV-40

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCIS-krPAM2

    Plasmid

    #25457

    Depositor

    Richard Mains

    Article

    Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

    Insert

    PAM2 (Pam Rat)
    

    Expression

    Mammalian

    Mutation

    Exon A deleted (exon A extends from nt 1475 to n…

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pGEX6P1-C. thermophilum LIC

    Plasmid

    #74780

    Purpose

    encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    dynein light intermediate chain (Chaetomium thermophilum)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAD06 K. lactis telomerase RNA hairpin

    Plasmid

    #61895

    Purpose

    Plasmid for run-off in vitro transcription of hairpin from the Kluyveromyces lactis telomerase RNA (nt 229-273)

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin (nt 229-273)
    

    Promoter

    T7

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only