168,951 results

1. Plasmid L5 attB::Pleft* mScarlet

   Plasmid

   #169410

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mScarlet

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   pLeft
   mScarlet proper
   

   Use

   Unspecified

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR-tdMCP-YFP

   Plasmid

   #99151

   Purpose

   Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   Dimeric MS2-coat binding protein
   

   Use

   Lentiviral

   Tags

   eYFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET29b-rhMOG

   Plasmid

   #225484

   Purpose

   Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

   Depositor

   John Pak

   Article

   Wu et al J Immunol Methods. 2024 Oct 13:113764. d

   Insert

   Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-29 (signal peptide) and 150…

   Promoter

   T7

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-PE2-His (IK1822)

   Plasmid

   #170103

   Purpose

   T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

   Depositor

   Keith Joung

   Article

   Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

   Insert

   bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
   

   Use

   CRISPR

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Bacteria codon-optimized nSpCas9 (H840A) & M-…

   Promoter

   T7

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-rab7 WT

   Plasmid

   #12605

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Tags

   GFP and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSAM_Ec

   Plasmid

   #102939

   Purpose

   Transposon mutagenesis and Tn-seq

   Depositor

   Matthew Mulvey

   Article

   Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

   Insert

   mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase
   

   Expression

   Bacterial

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIRES2-EGFP-p53 WT

   Plasmid

   #49242

   Purpose

   expresses human wt p53 and EGFP in mammalian cells

   Depositor

   Dylan Taatjes

   Article

   Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

   Insert

   p53 (TP53 Human)
   

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLQ10622

   Plasmid

   #239274

   Purpose

   Expresses PYL1-KIFC1* for the retrograde transport of RNA along microtubules via CRISPR-TO

   Depositor

   Stanley Qi

   Article

   Han et al Nature. 2025 Jul;643(8070):241-251. doi:

   Insert

   PYL1-BFP-KIFC1(aa125-673) (KIFC1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-KP1

   Plasmid

   #206504

   Purpose

   AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   KP1 capsid
   

   Use

   AAV

   Promoter

   p5

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-TRE-NpHR-EYFP

    Plasmid

    #171621

    Purpose

    TRE-NpHR-EYFP reporter

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TRE-NpHR-EYFP
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 Myc2- Hs TTP WT

    Plasmid

    #107008

    Purpose

    Expresses epitope-tagged wild-type human TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (ZFP36 Human)
    

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

    Plasmid

    #205555

    Purpose

    Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN24-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Arf4-GFP

    Plasmid

    #39556

    Depositor

    Paul Melancon

    Article

    Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

    Insert

    ADP ribosylaion factor 4 (ARF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pComb3XTT

    Plasmid

    #63891

    Purpose

    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    TT Fab
    

    Use

    Phage display

    Tags

    6xHis, HA tag, and geneIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLVX-hNINJ1-GFP

    Plasmid

    #208779

    Purpose

    Inducibly expresses human NINJ1 tagged with GFP

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (NINJ1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-GRAB_eCB2.0

    Plasmid

    #164604

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRDA_550

    Plasmid

    #203398

    Purpose

    All-in-one lentiviral expression of gRNA and EnAsCas12a

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Insert

    Enhanced Acidaminococcus Cas12a
    

    Use

    CRISPR and Lentiviral

    Promoter

    EF1a

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge