179,953 results
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Plasmid#122611Purposeexpress evoAPOBEC1-BE4max in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertevoAPOBEC1-BE4max
ExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (AAV9)
Viral prep#112005-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 (#112005). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 plasmid DNA. Synapsin-driven, axon-targeted GCaMP6s calcium sensor and mRuby3 expression. The mRuby3 will be a separate protein (not a fusion protein). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby3Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs GFP-LC3-RFP
Plasmid#117413PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.GFAP.Cre.WPRE.hGH
Plasmid#105550PurposeAAV expression of Cre from GFAP promoterDepositorHas serviceAAV5 and AAV PHP.eBInsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterGFAPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV4a-Flag-c-Myc
Plasmid#102625PurposeExpresses c-Myc in mammalian cellsDepositorAvailable sinceJan. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-Tre3G-29xGGGGCC-12xMS2
Plasmid#99149PurposeEncodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.DepositorInsert29xGGGGCC 12xMS2 hairpins
UseLentiviralExpressionMammalianPromoterTRE3GAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C321.∆A.opt
Bacterial strain#87359PurposeThis is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.DepositorBacterial resistanceGentamicinSpeciesSyntheticAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-FAST-p10
Plasmid#89152PurposeExpress p10-FAST in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNBV p10-FAST
ExpressionMammalianPromoterCAG promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUW-tetO-wtYAP
Plasmid#84009Purposeexpresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB401
Plasmid#74795PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable sinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ hnRNPK myc HIS
Plasmid#71662PurposeMammalian expression of hnRNPKDepositorAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ142
Plasmid#69294PurposeGolden Gate recipient and Gateway entry vector; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_iSH2-pMag(3x)-iRFP
Plasmid#67304PurposeBlue light dependent photoswitching systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiSH2-pMag(3x)-iRFP (PIK3R1 Synthetic, Human)
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMBP-MS2
Plasmid#65104Purposecan be used to isolate RNA binding complexesDepositorInsertMS2
TagsMBPExpressionBacterialMutationdouble mutation (V75Q and A81G) that prevents oli…PromotertacAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PX855
Plasmid#62887PurposeN-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 (aa 2-535)
UseCRISPRTagsFRB and NES PTK2ExpressionMammalianMutationAspartic acide 10 to Alanine (D10A)PromoterCBhAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-BETAipi
Plasmid#53277PurposeContains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF5-FRT-TagRFP-T-IFT88
Plasmid#61684PurposeMammalian expression of IFT88 fused with N terminal TagRFP.T tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIFT88 (Ift88 Mouse)
TagsTagRFP.TExpressionMammalianPromoterEF1aAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
TagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-hERG1a
Plasmid#53052PurposeExpresses alpha subunit of hERG1a K channel in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
ExpressionMammalianPromoterCMVAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1-puro U6 sgRNA BfuAI large stuffer
Plasmid#52628Purposelentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiencyDepositorInsertKanamycin cassette
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by