164,811 results

1. pcDNA3.2/DEST/hTfR-HA

   Plasmid

   #69610

   Purpose

   human transferrin receptor with an HA tag fused at the C-terminus

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   human transferrin receptor (TFRC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB533_15F11-sfGFP

   Plasmid

   #167526

   Purpose

   H4K20me1-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Sato et al J Mol Biol. 2016 Oct 9;428(20):3885-3902

   Insert

   15F11-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TVBB N-term-mTurquoise2

   Plasmid

   #169222

   Purpose

   Targeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mTurquoise2
   

   Use

   Targeting vector backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Rad18UBD

   Plasmid

   #119005

   Purpose

   Mammalian expression of the human Rad18 Ubiquitin binding domain and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Rad18
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-RNF169UBD

   Plasmid

   #119006

   Purpose

   Mammalian expression of the human RNF169 Ubiquitin binding domain and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   RNF169 (RNF169 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-tdTomato (AAV2)

   Viral Prep

   #28306-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   tdTomato (Cre-dependent)

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pMRX-IZU-LAMP1-mRuby3

   Plasmid

   #222586

   Purpose

   Stable expression of LAMP1-mRuby3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICS PM-ER Long P2A

   Plasmid

   #164111

   Purpose

   Detect the long Plasma membrane-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP PM-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONRp5-p2 GFP-NShroom3-iLID

   Plasmid

   #170976

   Purpose

   N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

   Depositor

   Miki Ebisuya

   Article

   Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

   Insert

   NShroom3 (Shroom3 Mouse)
   

   Use

   Gateway cloning

   Tags

   eGFP and iLID

   Expression

   Mutation

   From isoform 2, deleted amino acids 1389 - 1805,…

   Promoter

   Available since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid

    #170977

    Purpose

    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

    Depositor

    Miki Ebisuya

    Article

    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

    Insert

    CShroom3 (Shroom3 Mouse)
    

    Use

    Gateway cloning

    Tags

    SspB and mCherry

    Expression

    Mutation

    Isoform X2, deleted aminoacids 1 - 1388

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-RNF126

    Plasmid

    #138643

    Purpose

    Bacterial expression of GST-RNF126 fusion protein

    Depositor

    Jane McGlade

    Article

    Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

    Insert

    RNF126 (RNF126 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRosetta

    Plasmid

    #59700

    Purpose

    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

    Depositor

    John Doench, David Root

    Inserts

    Puro
    BlastR
    EGFP
    

    Use

    Lentiviral

    Tags

    2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Neo-Bam APC

    Plasmid

    #16507

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

    Plasmid

    #86414

    Purpose

    nuclear split GFP11 reporter without mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP11-PUS1
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1pr

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGem-PgpdA-Cre-TtrpC

    Plasmid

    #168784

    Purpose

    Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

    Insert

    Cre
    

    Use

    Cre/Lox and Synthetic Biology; Expression in fila…

    Tags

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330A-FBL/PITCh

    Plasmid

    #63671

    Purpose

    Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pICSL60004

    Plasmid

    #117519

    Purpose

    rbcS-E9 terminator Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GCTT:E9t:CGCT:BsaI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROP-sgRNA-MS2

    Plasmid

    #153457

    Purpose

    CROP-seq vector with mCherry and x2 MS2 loops in gRNA scaffold

    Depositor

    Wolf Reik

    Article

    Alda-Catalinas et al Cell Syst. 2020 Jul 22;11(1):25-41.e9. d

    Insert

    sgRNA empty backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB_iMYOD1_P2A_GFP_Puro

    Plasmid

    #168804

    Purpose

    PiggyBac transposon construct with doxycycline inducible MYOD1 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    MYOD1 (MYOD1 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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