We narrowed to 18,575 results for: MUT

1. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW240

   Plasmid

   #170791

   Purpose

   P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

   Depositor

   Chang Liu

   Article

   Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

   Insert

   p10B2-AT110
   

   Expression

   Yeast

   Promoter

   10B2 (P1 specific promoter)

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TR-Csy4-R115A/R119A

   Plasmid

   #80696

   Purpose

   Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-R115A/R119A
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Arg115 and Arg119 both mutated to Ala

   Promoter

   CBA

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. scFLARE2

   Plasmid

   #158700

   Purpose

   Neuronal expression of scFLARE2 component

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   scFLARE2
   

   Use

   AAV

   Tags

   HA, Flag, VP16

   Expression

   Mammalian

   Mutation

   Truncated form of TEV protease (220-242 amino aci…

   Promoter

   Syn

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28b-Cas9-His

   Plasmid

   #47327

   Purpose

   For in vitro expression and purification of Cas9 protein

   Depositor

   Alex Schier

   Article

   Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His

   Expression

   Bacterial

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE347

   Plasmid

   #153228

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-mitoTPNOX

   Plasmid

   #87854

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   mitoTPNOX
   

   Tags

   C-terminal FLAG tag with a linker and mitochondri…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_sfGPF 150TAG

   Plasmid

   #154765

   Purpose

   plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG

   Promoter

   EF1

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM233

    Plasmid

    #226655

    Purpose

    pcDNA3.1-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hprtSAGFP

    Plasmid

    #41594

    Purpose

    In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

    Insert

    SA-GFP reporter
    

    Use

    Mouse Targeting; Dsb repair reporter substrate

    Expression

    Mammalian

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGH020_sgRNA_G418-GFP

    Plasmid

    #85405

    Purpose

    hu6 driven sgRNA vector with G418 and GFP selectable markers

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    G418 resistance and GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRC-CMV-Rev1b

    Plasmid

    #204153

    Purpose

    Lentivirus helper plasmid coding for Rev

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    Rev
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pANAP

    Plasmid

    #48696

    Purpose

    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRS

    Depositor

    Peter Schultz

    Article

    Chatterjee et al J Am Chem Soc. 2013 Aug 28;135(34):12540

    Inserts

    8 copies of the amber suppressor tRNACUA-Leu
    AnapRS
    

    Expression

    Mammalian

    Mutation

    mutant E.coli leucyl synthetase specific for Anap…

    Promoter

    CMV and human H1 promoter

    Available Since

    Nov. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMax_mOrange

    Plasmid

    #177827

    Purpose

    Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-ExRai-CKAR(T/A)

    Plasmid

    #118410

    Purpose

    Negative-control mutant for ExRai-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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