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pOPINVHH_sortase_his

Plasmid

#210406

Purpose

For expression of VHH with C-terminal sortase and his tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

sortase-his6

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_his_myc

Plasmid

#210526

Purpose

For expression of VHH with C-terminal his and myc tags

Depositor

Ray Owens

Article

Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

Type

Empty backbone

Tags

His6-myc

Expression

Bacterial

Promoter

lac

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKSB-611U6-sgRNA3 AGAG AACA

Plasmid

#173673

Purpose

To clone and express a gRNA in Anopheles

Depositor

Eric Marois

Article

Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF17L

Plasmid

#35413

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17l (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
pK3FS-CYC1

Plasmid

#85779

Purpose

N-ICE plasmid pK3FS-CYC1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-CYC1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PKC beta II dNPS

Plasmid

#16383

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta II (Prkcb Mouse)

Tags

HA

Expression

Mammalian

Mutation

N-terminal pseudosubstrate domain deletion: aa 30…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
FXR1

Plasmid

#25341

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

FXR1_25 (FXR1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
Aahn

Plasmid

#112158

Purpose

A genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.

Depositor

Bradley Baker

Article

Sepehri Rad et al Sci Rep. 2018 May 2;8(1):6911. doi: 10.1

Insert

Aahn

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG

Plasmid

#188984

Purpose

Express chimadanin with TAG at 28 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 position

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSBDEST.H

Plasmid

#79464

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Hygromycin selection

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA3

Plasmid

#199729

Purpose

Golden Gate entry vector; 3rd gRNA under TaU3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU3-sgRNA3 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA4

Plasmid

#199730

Purpose

Golden Gate entry vector; 4th gRNA under ZmU6-C1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C1-sgRNA4 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA5

Plasmid

#199731

Purpose

Golden Gate entry vector; 5th gRNA under ZmU6-C3 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

ZmU6C3-sgRNA5 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA6

Plasmid

#199732

Purpose

Golden Gate entry vector; 6th gRNA under OsU6.1 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.1-sgRNA6 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA7

Plasmid

#199733

Purpose

Golden Gate entry vector; 7th gRNA under OsU6.2 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

OsU6.2-sgRNA7 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCBLsgRNA8

Plasmid

#199734

Purpose

Golden Gate entry vector; 8th gRNA under TaU6 promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

TaU6-sgRNA8 scaffold

Expression

Plant

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#176878

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP

Use

Luciferase

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-PMP-N-10

Plasmid

#55439

Purpose

Localization: Peroxisomes, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

PMP (PXMP2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-ZKSCAN1 nt 400-1782-mMALAT1_3' Sense

Plasmid

#60647

Purpose

Expresses the human ZKSCAN1 exon 2/3 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Paxillin-22

Plasmid

#55436

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Paxillin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pV1329

Plasmid

#111438

Purpose

Solo vector pV1326 +sgCgADE2

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgCgADE2

Use

CRISPR

Expression

Yeast

Available Since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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Thy1.2-NLS_HA_Dre-P2A-BFP

Plasmid

#51275

Purpose

Co-expression of the site-specific recombinase Dre and BFP under the control of the Thy1.2 expression cassette

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS_HA_Dre-P2A-BFP

Promoter

Thy1.2

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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mVermilion-C1

Plasmid

#105756

Purpose

Empty vector for creating N terminal mVermilion fusions

Depositor

Megan Rizzo

Article

Lee et al Elife. 2021 Oct 29;10. pii: 67858. doi:

Type

Empty backbone

Tags

mVermilion

Expression

Mammalian

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pISH-Satb1

Plasmid

#74357

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Satb1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

special AT-rich sequence binding protein 1 (Satb1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGL-MLKif3B

Plasmid

#13744

Depositor

Linda Wordeman

Article

Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

Insert

Motorless Kif3B (Kif3b Mouse)

Use

Cre/Lox

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2007

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCMB-AUTr

Plasmid

#61205

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128F

Plasmid

#61734

Purpose

Expresses human Y128F NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Mutation

Y128F

Promoter

Ef1a

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-APIC-Cas9

Plasmid

#121657

Purpose

gateway cloning destination vector for expressing enhancer driven Cas9

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NbALFA-TEV219

Plasmid

#201487

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbALFA-TEV219

Tags

TEV219

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MLS- mir-17-19b

Plasmid

#64089

Purpose

expresses a truncated version of mir-17-92 lacking miR-92

Depositor

Lin He

Article

Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

Insert

mir-17-19b (MIR17 Human)

Expression

Mammalian

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pWZL Hygro- TRF2 deltaB deltaM

Plasmid

#18013

Depositor

Titia de Lange

Article

Karlseder et al Science. 2002 Mar 29. 295(5564):2446-9.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
hGFAP-Roxed-Cre

Plasmid

#51263

Purpose

Expression of the Dre-dependent Roxed-Cre gene under the control of the human GFAP promoter

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

Roxed-Cre

Promoter

hGFAP

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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Showing: 10401 - 10440 of 26038 results

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