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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,851 results for: CRISPR-Cas9

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Showing: 5201 - 5220 of 13851 results

  1. pCBC-MT3T4

    Plasmid
    #50595
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T3, gRNA scaffold, U6-26t plus, OsU3p, T4
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50595-plasmid-map-sequence-id-75675

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  2. pMLM3705

    Plasmid
    #47754
    Purpose
    Expresses mammalian cell codon-optimized dCas9-VP64
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n
    Insert
    codon optimized dCas9-VP64
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and VP64
    Expression
    Mammalian
    Mutation
    D10A and H840A mutations in Cas9 (catalytically i…
    Promoter
    CMV
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47754-plasmid-map-sequence-id-68222

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  16. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54321-plasmid-map-sequence-id-84455

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  17. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid
    #64159
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA3 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64159-plasmid-map-sequence-id-107444

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  18. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid
    #64157
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA1 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64157-plasmid-map-sequence-id-107441

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  19. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid
    #64158
    Purpose
    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
    Depositor
    Moritoshi Sato
    Article
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Insert
    sgRNA2 for GAL4UAS-Luciferase reporter
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64158-plasmid-map-sequence-id-107442

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  20. pMG

    Plasmid
    #138264
    Purpose
    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Inserts
    eGFP flanked by iCas9 sites
    mCherry
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tef1
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138264-plasmid-map-sequence-id-273626

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Showing: 5201 - 5220 of 13851 results