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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 152,943 results for: DES

Showing: 5201 - 5220 of 152943 results

  1. mCit(S1h-S3h)3-gs

    Plasmid
    #228076
    Purpose
    Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228076-plasmid-map-sequence-id-454655

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  2. mCit(D1h-D3h)3-gs

    Plasmid
    #228088
    Purpose
    Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, D1h, D3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228088-plasmid-map-sequence-id-451399

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  3. mCit(S1-S3)1-SAH

    Plasmid
    #228073
    Purpose
    Mammalian expression of coiled coil segments (S1-S3)x1, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCitrine, S1, S3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228073-plasmid-map-sequence-id-451384

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  5. mCit(S1-S3)3-gs

    Plasmid
    #228070
    Purpose
    Mammalian expression of coiled coil segments (S1-S3)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCitrine, S1, S3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228070-plasmid-map-sequence-id-451165

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  6. TagBFP(S2-S4)3-gs

    Plasmid
    #228075
    Purpose
    Mammalian expression of coiled coil segments (S2-S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2, S4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228075-plasmid-map-sequence-id-451136

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  7. TagBFP(S2h)3(S4h)3-gs

    Plasmid
    #228077
    Purpose
    Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h, S4h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228077-plasmid-map-sequence-id-451130

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  10. pEndo-I-OnuI-TSM

    Plasmid
    #207954
    Purpose
    Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Insert
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in fu…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207954-plasmid-map-sequence-id-412663

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  12. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid
    #213401
    Purpose
    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2x35S
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213401-plasmid-map-sequence-id-422351

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  13. pRRL-VinculinTS-S1033A

    Plasmid
    #211835
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinTS-S1033A (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to alanine (S1033A),…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211835-plasmid-map-sequence-id-417709

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  14. pRRL-VinculinTS-S1033D

    Plasmid
    #211893
    Purpose
    Vinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinTS-S1033D (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211893-plasmid-map-sequence-id-419257

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  15. pRRL-VinculinCS-S1033A

    Plasmid
    #211894
    Purpose
    Vinculin conformation sensor (VinCS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinCS-S1033A (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to alanine (S1033A),…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211894-plasmid-map-sequence-id-417711

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  16. pRRL-VinculinCS-S1033D

    Plasmid
    #211895
    Purpose
    Vinculin conformation sensor (VinCS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12
    Insert
    VinculinCS-S1033D (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    mutated vinculin serine 1033 to aspartic acid (S1…
    Promoter
    CMV
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211895-plasmid-map-sequence-id-419255

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Showing: 5201 - 5220 of 152943 results